Stimulation of sulfated glycosaminoglycan synthesis by the tripeptide-copper complex Glycyl-L-histidyl-L-lysine-Cu2+
| Autor: | François-Xavier Maquart, J.P. Borel, Yanusz Wegrowski |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Molecular Sequence Data
Dermatan Sulfate Chondroitin sulfate B Tripeptide Sulfur Radioisotopes Tritium General Biochemistry Genetics and Molecular Biology Dermatan sulfate Glycosaminoglycan chemistry.chemical_compound Glucosamine medicine Humans Tissue Distribution Amino Acid Sequence Chondroitin sulfate General Pharmacology Toxicology and Pharmaceutics Fibroblast Cells Cultured Glycosaminoglycans Skin Chemistry Electrophoresis Cellulose Acetate General Medicine Heparan sulfate Fibroblasts Stimulation Chemical Culture Media medicine.anatomical_structure Biochemistry Oligopeptides |
| Zdroj: | Life Sciences. 51:1049-1056 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(92)90504-i |
| Popis: | Glycyl-L-histidyl-L-lysine-copper (II) complex (GHK-Cu) is a naturally occurring tripeptide with potential healing properties. We studied the effect of GHK-Cu on the synthesis of glycosaminoglycans (GAGs) by normal human fibroblasts in culture. Cells were incubated with 3H glucosamine and 35S sulfate and the radioactivity of isolated GAGs was determined. GHK-Cu induced a dose-dependent increase of the synthesis of total GAGs secreted into the culture medium and those associated with the cell layer. The effect of GHK-Cu was biphasic with a maximal stimulation at 10(-9) to 10(-8) M. At higher concentrations, the rate of synthesis returned progressively to that of control cultures. Electrophoretic analysis of the different GAG populations showed that GHK-Cu preferentially stimulated the synthesis of extracellular dermatan sulfate and cell layer associated heparan sulfate. No influence of GHK-Cu on the synthesis of hyaluronic acid was observed. GHK-Cu stimulation of GAG synthesis may be one of the phenomenons implicated in the wound healing properties of the peptide. |
| Databáze: | OpenAIRE |
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