Identification and characterization of sex‐biased and differentially expressed miRNAs in gonadal developments of the Chinese mitten crab, Eriocheir sinensis

Autor: Xin-yi Xiong, Xue Liu, Gao-Feng Qiu, Bi-Yun Luo, Xue-Ying He
Rok vydání: 2021
Předmět:
Zdroj: Molecular Reproduction and Development. 88:217-227
ISSN: 1098-2795
1040-452X
DOI: 10.1002/mrd.23459
Popis: MicroRNA (miRNA) is a posttranscriptional downregulator that plays a vital role in a wide variety of biological processes. In this study, we constructed five ovarian and testicular small RNA libraries using two somatic libraries as reference controls for the identification of sex-biased miRNAs and gonadal differentially expressed miRNAs (DEMs) of the Chinese mitten crab, Eriocheir sinensis. A total of 535 known and 243 novel miRNAs were identified, including 312 sex-biased miRNAs and 402 gonadal DEMs. KEGG pathway analysis showed that DEM target genes were statistically enriched in MAPK, Wnt, and GnRH signaling pathway, and so on. A number of the sex-biased miRNAs target genes associated with sex determination/differentiation, such as IAG, Dsx, Dmrt1, and Fem1, while others target the genes related to gonadal development, such as P450s, Wnt, Ef1, and Tra-2c. Dual-luciferase reporter assay in vitro further confirmed that miR-34 and let-7b can downregulate IAG expression, miR-9-5p, let-7d, let-7b, and miR-8915 can downregulate Dsx. Taken together, these data strongly suggest a potential role for the sex-biased miRNAs in sex determination/differentiation and gonadal development in the crab.
Databáze: OpenAIRE
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