Effects of the Herbicide 2,4,5-T on the Incorporation of 14C-Thymidine, 3H-Uridine and 3H-L-Leucine into L 929 Cells
| Autor: | Kristen Helgeland, L. Dragsnes |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Pharmacology
Facilitated diffusion 2 4 5-Trichlorophenoxyacetic Acid Cell Cell Fractionation Toxicology Uridine Mice chemistry.chemical_compound L Cells medicine.anatomical_structure chemistry Biochemistry Leucine medicine Protein biosynthesis Animals Chromatography Thin Layer Thymidine Incubation Nucleoside |
| Zdroj: | Acta Pharmacologica et Toxicologica. 36:103-112 |
| ISSN: | 0001-6683 |
| Popis: | A rapid inhibition of the incorporation of 3H-L-leucine, 3H-uridine and 14C-thymidine into the macromolecular fraction of L 929 cells was found when 2.25 mM 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) was added to the incubation medium. The reduced incorporation of 3H-uridine and 14C-thymidine could be explained by an inhibitory effect of 2,4,5-T on the uptake of these compounds into the acid-soluble precursor pool, whereas a direct effect on protein synthesis was indicated. Kinetic analysis of the uptake of uridine into the acid-soluble pool indicated that 2,4,5-T inhibited the facilitated transport of this nucleoside into the cell. The overall results are interpreted as indicating that 2,4,5-T induced alterations in the permeability of the cellular plasma membrane. |
| Databáze: | OpenAIRE |
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