Data from proteomic characterization of the role of Snail1 in murine mesenchymal stem cells and 3T3-L1 fibroblasts differentiation
| Autor: | J I Casal, Jean-Charles Sanchez, Marta Mendes, María F. López-Lucendo, Alberto Peláez-García, Rodrigo Barderas, A García de Herreros |
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| Rok vydání: | 2015 |
| Předmět: |
Proteomics
Mesenchymal cells Biology Mesenchymalcells Bioinformatics Tandem mass tag lcsh:Computer applications to medicine. Medical informatics Stable isotope labeling by amino acids in cell culture Transcriptionfactors Transcription factors Obesity ddc:576 Càncer lcsh:Science (General) Data Article Multidisciplinary Adipogenesis Mesenchymal stem cell Cèl·lules mare mesenquimàtiques Transfection Cell biology Isobaric labeling IL-17 Snail Differentiation lcsh:R858-859.7 Ectopic expression lcsh:Q1-390 |
| Zdroj: | Data in Brief, Vol. 4 (2015) pp. 606-613 Digital.CSIC. Repositorio Institucional del CSIC instname Data in Brief, Vol 4, Iss C, Pp 606-613 (2015) Data in Brief |
| ISSN: | 2352-3409 |
| DOI: | 10.1016/j.dib.2015.07.027 |
| Popis: | 8 p.-2 fig. The transcription factor (TF) Snail1 is a major inducer of the epithelial–mesenchymal transition (EMT) during embryonic development and cancer progression. Ectopic expression of Snail in murine mesenchymal stem cells (mMSC) abrogated their differentiation to osteoblasts or adipocytes. We used either stable isotopic metabolic labeling (SILAC) for 3T3-L1 cells or isobaric labeling with tandem mass tags (TMT) for mMSC stably transfected cells with Snail1 or control. We carried out a proteomic analysis on the nuclear fraction since Snail is a nuclear TF that mediates its effects mainly through the regulation of other TFs. Proteomics data have been deposited in ProteomeXchange via the PRIDE partner repository with the dataset identifiers PXD001529 and PXD002157 (Vizcaino et al., 2014) [1]. Data are associated with a research article published in Molecular and Cellular Proteomics (Pelaez-Garcia et al., 2015) This research was supported by grants to established research groups (AECC), BIO2012-31023 from the Spanish Ministry of Economy and Competitiveness, S2011/BMD-2344/ (Colomics2) from Comunidad de Madrid and Grant PRB2 (IPT13/0001 – ISCIII-SGEFI/FEDER) from ProteoRed-ISCIII. |
| Databáze: | OpenAIRE |
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