Molecular epidemiology of Corynebacterium pseudotuberculosis isolated from horses in California
| Autor: | Andrey Pereira Lage, Scott P. Carroll, Marcos Bryan Heinemann, Vasco Azevedo, Elaine Maria Seles Dorneles, Judy Edman, Sharon J. Spier, Dionei Joaquim Haas |
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| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Microbiology (medical) Genotype Corynebacterium pseudotuberculosis Biovar 030106 microbiology Biology Severity of Illness Index Microbiology CORYNEBACTERIUM California 03 medical and health sciences Intergenic region Genetics Animals Cluster Analysis Horses Molecular Biology Pathogen Genotyping Phylogeny Ecology Evolution Behavior and Systematics Molecular Epidemiology Genetic diversity Corynebacterium Infections Molecular epidemiology Genetic Variation biology.organism_classification DNA Fingerprinting Bacterial Typing Techniques Infectious Diseases DNA Intergenic Horse Diseases |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1567-1348 |
| DOI: | 10.1016/j.meegid.2016.12.011 |
| Popis: | Corynebacterium pseudotuberculosis biovar Equi is an important pathogen of horses. It is increasing in frequency in the United States, and is responsible for various clinical forms of infection, including external abscesses, internal abscesses of the abdominal or thoracic cavities, and ulcerative lymphangitis. The host/pathogen factors dictating the form or severity of infection are currently unknown. Our recent investigations have shown that genotyping C. pseudotuberculosis isolates using enterobacterial repetitive intergenic consensus (ERIC)-PCR is useful for understanding the evolutionary genetics of the species as well for molecular epidemiology studies. The aims of the present study were to assess (i) the genetic diversity of C. pseudotuberculosis strains isolated from horses in California, United States and (ii) the epidemiologic relationships among isolates. One hundred and seven C. pseudotuberculosis biovar Equi isolates from ninety-five horses, and two C. pseudotuberculosis biovar Ovis strains, C. pseudotuberculosis ATCC 19410T type strain and C. pseudotuberculosis 1002 vaccine strain, were fingerprinted using the ERIC 1 + 2-PCR. C. pseudotuberculosis isolated from horses showed a high genetic diversity, clustering in twenty-seven genotypes with a diversity index of 0.91. Minimal spanning tree showed four major clonal complexes with a pattern of temporal clustering. Strains isolated from the same horse showed identical ERIC 1 + 2-PCR genotype, with the exception of two strains isolated from the same animal that showed distinct genotypes, suggesting a co-infection. We found no strong genetic signals related to clinical form (including internal versus external infections). However, temporal clustering of genotypes was observed. |
| Databáze: | OpenAIRE |
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