Temporal alteration of spreading depression by the glycine transporter type-1 inhibitors NFPS and Org-24461 in chicken retina
| Autor: | Péter Mátyus, Szabolcs Kertesz, Laszlo G. Harsing, Geza Szabo, Szabolcs Udvari, György Lévay |
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| Rok vydání: | 2012 |
| Předmět: |
Agonist
N-Methylaspartate medicine.drug_class CHO Cells Neurotransmission Biology Kynurenic Acid Retina Glycine transporter Cricetulus Glycine Plasma Membrane Transport Proteins Cricetinae medicine Excitatory Amino Acid Agonists Animals Humans Molecular Biology General Neuroscience Chinese hamster ovary cell Cortical Spreading Depression Glutamate receptor Sarcosine Biochemistry Competitive antagonist Cortical spreading depression Glycine Neurology (clinical) Chickens Excitatory Amino Acid Antagonists Developmental Biology |
| Zdroj: | Brain research. 1492 |
| ISSN: | 1872-6240 |
| Popis: | We used isolated chicken retina to induce spreading depression by the glutamate receptor agonist N-methyl-d-aspartate. The N-methyl-d-aspartate-induced latency time of spreading depression was extended by the glycine(B) binding site competitive antagonist 7-chlorokynurenic acid. Addition of the glycine transporter type-1 inhibitors NFPS and Org-24461 reversed the inhibitory effect of 7-chlorokynurenic acid on N-methyl-d-aspartate-evoked spreading depression. The glycine uptake inhibitory activity of Org-24461, NFPS, and some newly synthesized analogs of NFPS was determined in CHO cells stably expressing human glycine transporter type-1b isoform. Compounds, which failed to inhibit glycine transporter type-1, also did not have effect on retinal spreading depression. These experiments indicate that the spreading depression model in chicken retina is a useful in vitro test to determine activity of glycine transporter type-1 inhibitors. In addition, our data serve further evidence for the role of glycine transporter type-1 in retinal neurotransmission and light processing. |
| Databáze: | OpenAIRE |
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