Effect of Sophora subprosrate polysaccharide on oxidative stress induced by PCV2 infection in RAW264.7 cells
| Autor: | Xue-Hong Shuai, Ting-Jun Hu, Dan Yin, Yun Zeng, Ying-Yi Wei, Zi-Jie Su |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Circovirus
Sophora Cell Survival Swine animal diseases Nitric Oxide Synthase Type II Nitric Oxide medicine.disease_cause Biochemistry Cell Line Nitric oxide Mice chemistry.chemical_compound Polysaccharides Structural Biology medicine Animals Molecular Biology Peroxidase chemistry.chemical_classification Reactive oxygen species Glutathione Disulfide biology Hydroxyl Radical Macrophages virus diseases General Medicine Glutathione biology.organism_classification Molecular biology Nitric oxide synthase Oxidative Stress chemistry Cell culture Myeloperoxidase biology.protein Reactive Oxygen Species Oxidative stress |
| Zdroj: | International Journal of Biological Macromolecules. 62:457-464 |
| ISSN: | 0141-8130 |
| Popis: | In this study, an oxidative stress model was first developed in a mouse macrophage cell line (RAW264.7 cells) by infecting the cells with porcine circovirus type 2 (PCV2). The regulatory effect of Sophora subprosrate polysaccharide (SSP) on PCV2-induced oxidative stress was investigated. The results showed that after infection with PCV2, reactive oxygen species (ROS) and nitric oxide (NO) production, myeloperoxidase (MPO) activity, and inducible nitric oxide synthase (iNOS) expression were significantly increased. Meanwhile, the ratio of reduced glutathione to oxidized glutathione (GSH/GSSG) and hydroxyl radical prevention capacity were greatly reduced. These data indicate successful creation of an oxidative stress model in RAW264.7 cells. A dramatic decrease in cell viability was observed in the cells exposed to oxidative stress compared to the control. When the cells were treated with SSP in concentrations of 100, 200 or 400 μg/mL post PCV2 infection, an increase in the GSH/GSSG ratio and hydroxyl radical prevention capacity was observed. We also observed decreased ROS and NO production, MPO activity, and iNOS expression in the infected cells. Our results demonstrated that PCV2 infection was able to induce oxidative stress in RAW264.7 cells and that SSP could reduce the negative effects resulting from the PCV2 infection. |
| Databáze: | OpenAIRE |
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