Spleen necrosis virus-derived C-type retroviral vectors for gene transfer to quiescent cells
| Autor: | Klaus Cichutek, Roger J. Pomerantz, Zahida Parveen, Anna Krupetsky, Ralph Dornburg, Martin Engelstädter |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
T-Lymphocytes
viruses Genetic enhancement Genetic Vectors Molecular Sequence Data Nuclear Localization Signals Biomedical Engineering Gene Products gag Mutagenesis (molecular biology technique) Bioengineering Biology Transfection Recombinant virus Applied Microbiology and Biotechnology Monocytes Virus Cell Line Jurkat Cells Necrosis Dogs Tumor Cells Cultured Animals Humans Amino Acid Sequence Vector (molecular biology) Cell Nucleus Extracellular Matrix Proteins Macrophages Genetic transfer Gene Transfer Techniques Flow Cytometry Virology Blotting Southern Cell culture Mutagenesis Site-Directed Molecular Medicine Gammaretrovirus Cell Division Spleen Nuclear localization sequence HeLa Cells Plasmids Biotechnology |
| Zdroj: | Nature Biotechnology. 18:623-629 |
| ISSN: | 1546-1696 1087-0156 |
| Popis: | Gene therapy applications of retroviral vectors derived from C-type retroviruses have been limited to introducing genes into dividing target cells. Here, we report genetically engineered C-type retroviral vectors derived from spleen necrosis virus (SNV), which are capable of infecting nondividing cells. This has been achieved by introducing a nuclear localization signal (NLS) sequence into the matrix protein (MA) of SNV by site-directed mutagenesis. This increased the efficiency of infecting nondividing cells and was sufficient to endow the virus with the capability to efficiently infect growth-arrested human T lymphocytes and quiescent primary monocyte-derived macrophages. We demonstrate that this vector actively penetrates the nucleus of a target cell, and has potential use as a gene therapy vector to transfer genes into nondividing cells. |
| Databáze: | OpenAIRE |
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