Sex Differences in Kidney Function and Metabolism Assessed Using Hyperpolarized [1-13C]Pyruvate Interleaved Spectroscopy and Nonspecific Imaging
| Autor: | Per Mose Nielsen, Yibo Wen, Haiyun Qi, Christian Østergaard Mariager, Christoffer Laustsen, Hans Stødkilde-Jørgensen, Lotte Bonde Bertelsen |
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| Rok vydání: | 2020 |
| Předmět: |
Male
kidney spectroscopy medicine.medical_specialty Renal function Kidney weight Internal medicine Pyruvic Acid medicine sex Animals Radiology Nuclear Medicine and imaging Rats Wistar Advances in Brief hyperpolarization Sex Characteristics Kidney medicine.diagnostic_test business.industry renal metabolism Spectrum Analysis Magnetic resonance imaging Metabolism Magnetic Resonance Imaging Rats Oxygen tension Endocrinology medicine.anatomical_structure BSSFP Blood oxygenation Female Functional status business MRI |
| Zdroj: | Wen, Y, Qi, H, Østergaard Mariager, C, Mose Nielsen, P, Bonde Bertelsen, L, Stødkilde-Jørgensen, H & Laustsen, C 2020, ' Sex Differences in Kidney Function and Metabolism Assessed Using Hyperpolarized [1-13C]Pyruvate Interleaved Spectroscopy and Nonspecific Imaging ', Tomography-A Journal for Imaging Research, vol. 6, no. 1, pp. 5-13 . https://doi.org/10.18383/j.tom.2020.00022 Tomography |
| ISSN: | 2379-139X |
| DOI: | 10.18383/j.tom.2020.00022 |
| Popis: | Metabolic sex differences have recently been shown to be particularly important in tailoring treatment strategies. Sex has a major effect on fat turnover rates and plasma lipid delivery in the body. Differences in kidney structure and transporters between male and female animals have been found. Here we investigated sex-specific renal pyruvate metabolic flux and whole-kidney functional status in age-matched healthy Wistar rats. Blood oxygenation level–dependent and dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) were used to assess functional status. Hyperpolarized [1-13C]pyruvate was used to assess the metabolic differences between male and female rats. Female rats had a 41% ± 3% and 41% ± 5% lower absolute body and kidney weight, respectively, than age-matched male rats. No difference was seen between age-matched male and female rats in the kidney-to-body weight ratio. A 56% ± 11% lower lactate production per mL/100 mL/min was found in female rats than in age-matched male rats measured by hyperpolarized magnetic resonance and DCE MRI. Female rats had a 33% ± 11% higher glomerular filtration rate than age-matched male rats measured by DCE MRI. A similar renal oxygen tension (T2*) was found between age-matched male and female rats as shown by blood oxygenation level–dependent MRI. The results were largely independent of the pyruvate volume and the difference in body weight. This study shows an existing metabolic difference between kidneys in age-matched male and female rats, which indicates that sex differences need to be considered when performing animal experiments. |
| Databáze: | OpenAIRE |
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