NEC1, a novel gene, highly expressed in nectary tissue of Petunia hybrida
| Autor: | Le-Min Zhang, G. J. Wullems, Tineke Creemers-Molenaar, Jeroen Peters, Ellen Dahlhaus, Marco Busscher, Gerco C. Angenent, Ya-Xin Ge, P.E. Wittich, Martin M. Kater, John Franken |
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| Rok vydání: | 2001 |
| Předmět: |
DNA
Complementary DNA Plant Recombinant Fusion Proteins Molecular Sequence Data Gene Expression Plant Science Biology Genes Plant Petunia Gene expression Botany Genetics Nectar Tissue Distribution Northern blot Amino Acid Sequence Promoter Regions Genetic Solanaceae Plant Proteins Differential display Base Sequence fungi food and beverages Membrane Proteins Nectar secretion Starch Cell Biology biology.organism_classification Cell biology Hybridization Genetic Ectopic expression Phloem |
| Zdroj: | The Plant journal : for cell and molecular biology. 24(6) |
| ISSN: | 0960-7412 |
| Popis: | To study the molecular regulation of nectary development, we cloned NEC1, a gene predominantly expressed in the nectaries of Petunia hybrida, by using the differential display RT-PCR technique. The secondary structure of the putative NEC1 protein is reminiscent of a transmembrane protein, indicating that the protein is incorporated into the cell membrane or the cytoplast membrane. Immunolocalization revealed that NEC1 protein is present in the nectaries. Northern blot analyses showed that NEC1 is highly expressed in nectary tissue and weakly in the stamen. GUS expression driven by the NEC1 promoter revealed GUS activity in the outer nectary parenchyma cells, the upper part of the filament and the anther stomium. The same expression pattern was observed in Brassica napus. GUS expression was observed as blue spots on the surface of very young nectaries that do not secrete nectar and do accumulate starch. GUS expression was highest in open flowers in which active secretion of nectar and starch hydrolysis had taken place. Ectopic expression of NEC1 resulted in transgenic plants that displayed a phenotype with leaves having 3-4 times more phloem bundles in mid-veins than the wild-type Petunia. The possible role of NEC1 gene in sugar metabolism and nectar secretion is discussed. |
| Databáze: | OpenAIRE |
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