PAS-1, a protein from Ascaris suum, modulates allergic inflammation via IL-10 and IFN-γ, but not IL-12
| Autor: | C.A. Araújo, Adenir Perini, M. F. Macedo-Soares, Mahasti Sahihi de Macedo, Milton A. Martins |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Eotaxin Immunology Immunoglobulin E Biochemistry Immunoglobulin G Allergic inflammation Interferon-gamma Mice Cell Movement Leukocytes medicine Animals Immunology and Allergy Interferon gamma Molecular Biology Ascaris suum Mice Knockout biology Helminth Proteins Hematology respiratory system Eosinophil biology.organism_classification Interleukin-12 Interleukin-10 Mice Inbred C57BL Interleukin 10 medicine.anatomical_structure Antibody Formation biology.protein Bronchial Hyperreactivity medicine.drug |
| Zdroj: | Cytokine. 44:335-341 |
| ISSN: | 1043-4666 |
| DOI: | 10.1016/j.cyto.2008.09.005 |
| Popis: | Helminths and their products have a profound immunomodulatory effect upon the inductive and effector phases of inflammatory responses, including allergy. We have demonstrated that PAS-1, a protein isolated from Ascaris suum worms, has an inhibitory effect on lung allergic inflammation due to its ability to down-regulate eosinophilic inflammation, Th2 cytokine release and IgE antibody production. Here, we investigated the role of IL-12, IFN-gamma and IL-10 in the PAS-1-induced inhibitory mechanism using a murine model of asthma. Wild type C57BL/6, IL-12(-/-), IFN-gamma(-/-) and IL-10(-/-) mice were immunized with PAS-1 and/or OVA and challenged with the same antigens intranasally. The suppressive effect of PAS-1 was demonstrated on the cellular influx into airways, with reduction of eosinophil number and eosinophil peroxidase activity in OVA+PAS-1-immunized wild type mice. This effect well correlated with a significant reduction in the levels of IL-4, IL-5, IL-13 and eotaxin in BAL fluid. Levels of IgE and IgG1 antibodies were also impaired in serum from these mice. The inhibitory activity of PAS-1 was also observed in IL-12(-/-) mice, but not in IFN-gamma(-/-) and IL-10(-/-) animals. These data show that IFN-gamma and IL-10, but not IL-12, play an important role in the PAS-1 modulatory effect. |
| Databáze: | OpenAIRE |
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