The PPARγ agonist rosiglitazone promotes the induction of brite adipocytes, increasing β-adrenoceptor-mediated mitochondrial function and glucose uptake
| Autor: | Bronwyn A Evans, Tore Bengtsson, Jon Merlin, Mohsen Pakzad, Roger J. Summers, Dana S. Hutchinson, Nodi Dehvari, Jie Gao, Masaaki Sato, Cameron J. Nowell, Richard Fahey |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Agonist Male medicine.medical_specialty medicine.drug_class Glucose uptake Adipocytes White Primary Cell Culture Adipose tissue Stimulation Biology Rosiglitazone 03 medical and health sciences Mice 0302 clinical medicine Oxygen Consumption Internal medicine Brown adipose tissue medicine Cyclic AMP Animals Hypoglycemic Agents Adipocytes Beige Receptor Uncoupling Protein 1 Glucose Transporter Type 1 Glucose Transporter Type 4 Biological Transport Cell Biology Thermogenin Mitochondria PPAR gamma 030104 developmental biology medicine.anatomical_structure Endocrinology Adipocytes Brown Glucose Gene Expression Regulation Organ Specificity Receptors Adrenergic beta-3 Thiazolidinediones 030217 neurology & neurosurgery medicine.drug Signal Transduction |
| Zdroj: | Cellular signalling. 42 |
| ISSN: | 1873-3913 |
| Popis: | Recruitment and activation of brite (or beige) adipocytes has been advocated as a potential avenue for manipulating whole-body energy expenditure. Despite numerous studies illustrating the differences in gene and protein markers between brown, brite and white adipocytes, there is very little information on the adrenergic regulation and function of these brite adipocytes. We have compared the functional (cyclic AMP accumulation, oxygen consumption rates, mitochondrial function, glucose uptake, extracellular acidification rates, calcium influx) profiles of mouse adipocytes cultured from three contrasting depots, namely interscapular brown adipose tissue, and inguinal or epididymal white adipose tissues, following chronic treatment with the peroxisome proliferator-activated receptor γ (PPARγ) agonist rosiglitazone. Prototypical brown adipocytes readily express β3-adrenoceptors, and β3-adrenoceptor stimulation increases cyclic AMP accumulation, oxygen consumption rates, mitochondrial function, glucose uptake, and extracellular acidification rates. Treatment of brown adipocytes with rosiglitazone increases uncoupling protein 1 (UCP1) levels, and increases β3-adrenoceptor mitochondrial function but does not affect glucose uptake responses. In contrast, inguinal white adipocytes only express UCP1 and β3-adrenoceptors following rosiglitazone treatment, which results in an increase in all β3-adrenoceptor-mediated functions. The effect of rosiglitazone in epididymal white adipocytes, was much lower compared to inguinal white adipocytes. Rosiglitazone also increased α1-adrenoceptor mediated increases in calcium influx and glucose uptake (but not mitochondrial function) in inguinal and epididymal white adipocytes. In conclusion, the PPARγ agonist rosiglitazone promotes the induction and function of brite adipocytes cultured from inguinal and epididymal white adipose depots. |
| Databáze: | OpenAIRE |
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