Effects of Neonatal Caffeine Administration on Vessel Reactivity in Adult Mice
| Autor: | Ajay Pratap Singh, Huamei Wang, Sylvia F. Gugino, Lori Nielsen, Praveen Chandrasekharan, Sara K. Berkelhamer, Vasantha H.S. Kumar |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Urinary system Intraperitoneal injection Vasodilation Blood Pressure 03 medical and health sciences chemistry.chemical_compound Feces Mice Norepinephrine 0302 clinical medicine Sex Factors Corticosterone Risk Factors Stress Physiological Internal medicine medicine.artery Caffeine medicine Animals Chronic stress Aorta 030219 obstetrics & reproductive medicine business.industry Obstetrics and Gynecology Endocrinology chemistry Animals Newborn 8-Hydroxy-2'-Deoxyguanosine Vasoconstriction Pediatrics Perinatology and Child Health Hypertension Female medicine.symptom business |
| Zdroj: | American journal of perinatology. 38(12) |
| ISSN: | 1098-8785 |
| Popis: | Objective The effects of neonatal caffeine therapy in adults born preterm are uncertain. We studied the impact of neonatal caffeine on systemic blood pressure, vessel reactivity, and response to stress in adult mice. Study Design Mice pups were randomized to caffeine (20 mg/kg/d) or saline by intraperitoneal injection for 10 days after birth. We performed tail-cuff BP (8/12 weeks), urinary 8-hydroxydeoxyguanosine and fecal corticosterone (14 weeks), and vessel reactivity in aortic rings (16 weeks) in adult mice. Results No differences were noted in systolic, diastolic, and mean blood pressures between the two groups at 8 and 12 weeks of age. However, norepinephrine-induced vasoconstriction was substantially higher in aortic rings in CAF-treated male mice. More significant vasodilator responses to nitric oxide donors in aortic rings in female mice may suggest gender-specific effects of caffeine. Female mice exposed to caffeine had significantly lower body weight over-time. Caffeine-treated male mice had substantially higher fecal corticosterone and urinary 8-hydroxydeoxyguanosine at 14 weeks, suggestive of chronic stress. Conclusion We conclude sex-specific vulnerability to the heightened vascular tone of the aorta in male mice following neonatal caffeine therapy. Altered vessel reactivity and chronic stress in the presence of other risk factors may predispose to the development of systemic hypertension in adults born preterm. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|