Genetic diversity of Rhizoctonia solani associated with potato tubers in France
| Autor: | Véronique Edel-Hermann, Christian Steinberg, Marie Fiers, Catherine Chatot, Cécile Héraud, Nadine Gautheron, Yves Le Hingrat, K. Bouchek-Mechiche |
|---|---|
| Přispěvatelé: | Microbiologie, Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Germicopa, Fédération Nationale des Producteurs de Plants de Pomme de Terre (FN3PT), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST |
| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
0301 basic medicine internal transcribed spacer polymorphic site Physiology Molecular Sequence Data anastomosis group Biology 010603 evolutionary biology 01 natural sciences amplified fragment length polymorphism Rhizoctonia Rhizoctonia solani 03 medical and health sciences Genetic variation Botany DNA Ribosomal Spacer Genetics Internal transcribed spacer Amplified Fragment Length Polymorphism Analysis Molecular Biology Ecology Evolution Behavior and Systematics [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology Phylogeny Solanum tuberosum 2. Zero hunger Genetic diversity Phylogenetic tree Base Sequence food and beverages Genetic Variation Cell Biology General Medicine 030108 mycology & parasitology Ribosomal RNA biology.organism_classification elongation factor DNA profiling potato Amplified fragment length polymorphism France anastamose |
| Zdroj: | Mycologia Mycologia, Mycological Society of America, 2011, 103 (6), pp.1230-1244. ⟨10.3852/10-231⟩ Mycologia, 2011, 103 (6), pp.1230-1244. ⟨10.3852/10-231⟩ |
| ISSN: | 0027-5514 |
| DOI: | 10.3852/10-231⟩ |
| Popis: | Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; The soilborne fungus Rhizoctonia solani is a pathogen of many plants and causes severe damage in crops around the world. Strains of R. solani from the anastomosis group (AG) 3 attack potatoes, leading to great yield losses and to the downgrading of production. The study of the genetic diversity of the strains of R. solani in France allows the structure of the populations to be determined and adapted control strategies against this pathogen to be established. The diversity of 73 French strains isolated from tubers grown in the main potato seed production areas and 31 strains isolated in nine other countries was assessed by phylogenetic analyses of (i) the internal transcribed spacer sequences (ITS1 and ITS2) of ribosomal RNA (rRNA), (ii) a part of the gene tef-1a and (iii) the total DNA fingerprints of each strain established by amplified fragment length polymorphism (AFLP). The determination of the AGs of R. solani based on the sequencing of the ITS region showed three different AGs among our collection (60 AG 3 PT, 8 AG 2-1 and 5 AG 5). Grouping of the strains belonging to the same AG was confirmed by sequencing of the gene tef-1a used for the first time to study the genetic diversity of R. solani. About 42% of ITS sequences and 72% of tef-1a sequences contained polymorphic sites, suggesting that the cells of R. solani strains contain several copies of ITS and the tef-1a gene within the same nucleus or between different nuclei. Phylogenetic trees showed a greater genetic diversity within AGs in tef-1a sequences than in ITS sequences. The AFLP analyses showed an even greater diversity among the strains demonstrating that the French strains of R. solani isolated from potatoes were not a clonal population. Moreover there was no relationship between the geographical origins of the strains or the variety from which they were isolated and their genetic diversity. |
| Databáze: | OpenAIRE |
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