Genome shuffling of Colletotrichum lini for improving 3β,7α,15α-trihydroxy-5-androsten-17-one production from dehydroepiandrosterone
| Autor: | Zhenghong Xu, Sun Jin, Xiaomei Zhang, Jin-Song Shi, Ni Yu, Hui Li |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Colletotrichum lini Cytochrome Dehydroepiandrosterone Bioengineering 01 natural sciences Applied Microbiology and Biotechnology law.invention 03 medical and health sciences Cytochrome P-450 Enzyme System law 010608 biotechnology Colletotrichum Genetics Androstenols biology Substrate (chemistry) DNA Shuffling Protoplast biology.organism_classification Enzyme assay 030104 developmental biology Biochemistry Yield (chemistry) biology.protein Recombinant DNA Genome Fungal Biotechnology |
| Zdroj: | Journal of industrial microbiologybiotechnology. 44(6) |
| ISSN: | 1476-5535 |
| Popis: | 3β,7α,15α-Trihydroxy-5-androsten-17-one (7α,15α-diOH-DHEA) is a key intermediate of the novel oral contraceptive Yasmin. It can be catalyzed from dehydroepiandrosterone (DHEA) through Colletotrichum lini. Improvement of 7α,15α-diOH-DHEA production was performed through recursive protoplast fusion of C. lini ST in a genome shuffling format. 7α,15α-diOH-DHEA yield of the best performing recombinant C. lini ST-F307 reached 6.08 g/L from 10 g/L DHEA, and this was 94.9% higher than that of the initial C. lini ST strain. Through optimized conditions, the 7α,15α-diOH-DHEA yield was increased to 9.32 g/L from 12 g/L DHEA, with 1.5% ethanol as cosolvent. This is the highest reported substrate concentration and 7α,15α-diOH-DHEA production with one-step substrate addition. Moreover, C. lini ST-F307 showed high P450 enzyme activity and gene transcript levels of several cytochrome P450s, and this might contribute to the enhancement of 7α,15α-diOH-DHEA production. Genome shuffling was an efficient approach to breed high-yield strains. |
| Databáze: | OpenAIRE |
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