Disruption of kynurenine pathway reveals physiological importance of tryptophan catabolism in Henosepilachna vigintioctopunctata
Autor: | Ping Xu, Wei-Nan Kang, Lin Jin, Ahmad Ali Anjum, Guo-Qing Li, Long-Ji Ze, Jian-Jian Wu |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Kynurenine pathway Clinical Biochemistry Henosepilachna vigintioctopunctata Biology Biochemistry 03 medical and health sciences Phenothiazines Phototaxis Animals Ommochrome Kynurenine Negative phototaxis Gene knockdown Eye Color 030102 biochemistry & molecular biology Catabolism fungi Organic Chemistry Tryptophan biology.organism_classification Biosynthetic Pathways Cell biology Coleoptera 030104 developmental biology Larva Insect Proteins Locomotion |
Zdroj: | Amino Acids. 53:1091-1104 |
ISSN: | 1438-2199 0939-4451 |
DOI: | 10.1007/s00726-021-03009-4 |
Popis: | Kynurenine pathway is critically important to catabolize tryptophan, to produce eye chromes, and to protect nervous system in insects. However, several issues related to tryptophan degradation remain to be clarified. In the present paper, we identified three genes (karmoisin, vermilion and cardinal) involved in kynurenine pathway in Henosepilachna vigintioctopunctata. The karmoisin and cardinal were highly expressed in the pupae and adults having compound eyes. Consistently, high-performance liquid chromatography result showed that three ommochrome peaks were present in adult heads rather than bodies (thoraces, legs, wings and abdomens). RNA interference (RNAi)-aided knockdown of vermilion caused accumulation of tryptophan in both adult heads and bodies, disappearance of ommochromes in the heads and a complete loss of eye color in both pupae and adults. Depletion of cardinal brought about excess of 3-hydroxykynurenine and insufficient ommochromes in the heads and decolored eyes. RNAi of karmoisin resulted in a decrease in ommochromes in the heads, and a partial loss of eye color. Moreover, a portion of karmoisin-, vermilion- or cardinal-silenced adults exhibited negative phototaxis, whereas control beetles showed positive phototaxis. Furthermore, dysfunctions of tryptophan catabolism impaired climbing ability. Our findings clearly illustrated several issues related to kynurenine pathway and provided a new insight into the physiological importance of tryptophan catabolism in H. vigintioctopunctata. |
Databáze: | OpenAIRE |
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