The impact of cytokine gene polymorphisms on the outcome of HLA matched sibling hematopoietic stem cell transplantation
| Autor: | Azza M. Kamel, Gamal T. A. Ebid, Eman R. Radwan, Mostafa F. Mohammed Saleh, Abdallah Gameel, Raafat Abdelfattah |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Adolescent Genotype medicine.medical_treatment Immunology CD34 Graft vs Host Disease Hematopoietic stem cell transplantation Human leukocyte antigen Biochemistry Polymorphism Single Nucleotide Pathogenesis 03 medical and health sciences Young Adult 0302 clinical medicine HLA Antigens medicine Immunology and Allergy Humans Transplantation Homologous Child Molecular Biology business.industry Incidence (epidemiology) Siblings Hematopoietic Stem Cell Transplantation Interleukin Hematology Middle Aged Interleukin 10 030104 developmental biology Phenotype Child Preschool Cytokines Female business 030215 immunology |
| Zdroj: | Cytokine. 110 |
| ISSN: | 1096-0023 |
| Popis: | Graft-versus-host disease (GVHD) is the major complication of allogeneic hematopoietic stem cell transplantation (HSCT); cytokines are recognized as important mediators in its pathogenesis. In this study we investigated the role of cytokine gene polymorphisms on HSCT outcome. A total of 106 patient and 98 donors were genotyped by polymerase chain reaction sequence specific primers (PCR-SSP) based assay for tumor necrosis factor-α−308 (TNFα -308), interleukin (IL)-6-174, IL-10-1082, −819, −592, Interferon-γ+874 (IFN-γ+874), and transforming growth factor-β1 (TGF-β1) codon10 and 25 polymorphisms. Except one in each category, all patients and donors were TNFα -308 high producers and the majority were IL-6-174 high producers (93.3% and 90.8% respectively); a pattern that would alleviate any potential biological impact. Patient's IFN-γ+874 showed significant association with the development of chronic GVHD. Patients with IFN-γ +874 high producer showed an 8 folds likelihood to develop chronic GVHD as compared to those with IFN-γ+874 low producer predicted phenotype (95% CI: 1.59-40.2, p = 0.01). Patient's TGFβ1-codon 10 and 25 high/intermediate producers showed a lower incidence of acute GVHD though it did not achieve statistical significance (p = 0.065) on account of the low frequency of this genotype in our patients and donors (11.4 and 8.2% respectively). Other factors contributing to risk of GVHD included older age for both acute and chronic (p = 0.01 and 0.02 respectively) with age 24 as the best discriminating cutoff; CD34+ cell dose for chronic GVHD (p = 0.045) with a dose of 8 × 106/kg as the best discriminating cutoff; and conditioning regimen with Flu/Bu associated with the lowest incidence of acute GVHD (p = 0.003) and no impact on chronic GVHD. In conclusion the current study further indicates a potential role of some cytokine gene polymorphisms in the development of GVHD. The relative distribution of high and low producer genotypes in different ethnic groups contributes to their biological impact in different populations. |
| Databáze: | OpenAIRE |
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