Lytic effect ofVibrio cholerae elastase on Gram-positive and-negative bacteria
| Autor: | K. K. Tripathi, K. G. Gupta, Meenakshi Gupta, Maninder Kaur |
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| Rok vydání: | 1990 |
| Předmět: |
Hot Temperature
Gram-negative bacteria Pancreatic Elastase biology Gram-positive bacteria Elastase Proteus vulgaris General Medicine Hydrogen-Ion Concentration Gram-Positive Bacteria biology.organism_classification medicine.disease_cause Microbiology Bacteriolysis Vibrionaceae Vibrio cholerae Staphylococcus aureus Gram-Negative Bacteria medicine Bacteria |
| Zdroj: | Folia Microbiologica. 35:183-189 |
| ISSN: | 1874-9356 0015-5632 |
| DOI: | 10.1007/bf02820483 |
| Popis: | Elastase of Vibrio cholerae caused the lysis of freshly grown cells of Gram-negative (Pseudomonas aeruginosa, Proteus vulgaris, Salmonella paratyphi A and Klebsiella pneumoniae) bacteria. Gram-positive (Staphylococcus aureus and S. epidermidis) organisms were resistant to this enzyme. Heat killed and lyophilized Gram-positive and -negative bacteria (except S. aureus and S. epidermidis) showed higher sensitivity to elastase. Both Gram-negative and -positive bacteria were lyzed maximally by elastase at pH 8.0. At this pH, lytic activity of elastase was maximum in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and cacodylate buffers. |
| Databáze: | OpenAIRE |
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