Tritium isotope effect in high-performance liquid chromatography of eicosanoids
| Autor: | U. Hoi Do, D.G. Ahern, Y. Hong, P. Tam, S.-L. Lo, J. Iles, T. Rosenberger |
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| Rok vydání: | 1994 |
| Předmět: |
Chromatography
Molecular Structure Isotope Hydrogen Chemistry Elution Clinical Biochemistry chemistry.chemical_element hemic and immune systems Cell Biology Reversed-phase chromatography respiratory system Tritium High-performance liquid chromatography Eicosanoid Kinetic isotope effect cardiovascular system Eicosanoids lipids (amino acids peptides and proteins) tissues Chromatography High Pressure Liquid |
| Zdroj: | Prostaglandins, Leukotrienes and Essential Fatty Acids. 50:335-338 |
| ISSN: | 0952-3278 |
| DOI: | 10.1016/0952-3278(94)90243-7 |
| Popis: | A significant difference in retention time between unlabeled and the corresponding multi-tritium-labeled eicosanoid has been observed in the high performance liquid chromatography (HPLC) analysis of 11 eicosanoids. Variations in retention time range from 3-7%, depending on the separation conditions as well as the number and position of the tritium substitution. Multi-tritium-labeled eicosanoids were eluted earlier than the corresponding unlabeled eicosanoid in reversed phase HPLC, whereas no isotope effect was seen with 14C- and 3H2-eicosanoids. Considerations must be given to this tritium isotope effect whenever both multi-tritium-labeled and unlabeled eicosanoids are used for HPLC cochromatography or recovery studies. |
| Databáze: | OpenAIRE |
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