In-Vitro Use of 5-ALA for Photodynamic Therapy in Pediatric Brain Tumors
| Autor: | Christian Ewelt, Andrei Nemes, Juliane Schroeteler, Volker Senner, Jana Dondrop, Stephanie Schipmann, Walter Stummer, Michael Schwake |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Programmed cell death
Necrosis Cell Survival medicine.medical_treatment Brain tumor Protoporphyrins Photodynamic therapy Apoptosis 03 medical and health sciences 0302 clinical medicine Glioma Cell Line Tumor medicine Humans Viability assay Child Medulloblastoma Photosensitizing Agents Cell Death business.industry Brain Neoplasms medicine.disease Levulinic Acids Photochemotherapy 030220 oncology & carcinogenesis Cancer research Surgery Neurology (clinical) medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | Neurosurgery. 83(6) |
| ISSN: | 1524-4040 |
| Popis: | BACKGROUND Light irradiation (635 nm) of cells containing protoporphyrin IX (PPIX) after 5- aminolevulinic acid (5-ALA) pretreatment causes cell death via different pathways including apoptosis and necrosis, as previously demonstrated for malignant glioma cells. OBJECTIVE To elucidate whether various malignant pediatric brain tumors, which have been shown to accumulate PPIX, would also be susceptible to photodynamic therapy (PDT). METHODS Medulloblastoma (DAOY, UW228), pNET (PFSK-1), and rhabdoid tumor (BT16) cell lines were incubated with 5-ALA in variable concentrations for 4 h. Consequently, cells were irradiated by 635 nm diode laser light. After 12 h, cell viability was measured by WST-1 testing and these results were compared to control cells incubated with 5-ALA without irradiation or irradiation only without prior incubation with 5-ALA. RESULTS We demonstrated significant cell death in malignant pediatric tumor cells after incubation with 5-ALA and laser irradiation in comparison to control groups. In all cell lines, we noticed significant cell death above a 5-ALA concentration of 50 μg/ml (P < .05). Neither 5-ALA incubation alone nor irradiation alone caused cell death. DAOY and PFSK cell lines were more susceptible than UW228 and BT16 cells. CONCLUSION We conclude that PDT causes cell death with higher PPIX concentrations after exposure to 5-ALA in vitro in accordance to similar studies with glioma cells. This indicates that PDT might be feasible for eliminating brain tumor cells in malignant pediatric brain tumors. Additionally, we noticed a dependency between fluorescence intensity and death rates. |
| Databáze: | OpenAIRE |
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