Evaluation of thrombus detection in a rabbit model using a technetium-99M-labelled anti-fibrin monoclonal antibody
| Autor: | P.G. Bundesen, G.R. Boniface, L. J. Milner, A. R. J. Weedon, K. Z. Walker, Fook-Thean Lee, George J. Bautovich, D.B. Rylatt |
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| Rok vydání: | 1992 |
| Předmět: |
Male
0301 basic medicine medicine.drug_class Immunology Fibrinogen Monoclonal antibody Fibrin Epitope Fibrin Fibrinogen Degradation Products Immunoglobulin Fab Fragments 03 medical and health sciences 0302 clinical medicine Antibody Specificity medicine Animals Immunology and Allergy Tissue Distribution Thrombus biology Chemistry Technetium Thrombosis Cell Biology medicine.disease Fibrin Monomer Molecular biology 030104 developmental biology Radioimmunodetection Evaluation Studies as Topic Immunoglobulin G biology.protein Female Rabbits Antibody Technetium-99m 030215 immunology medicine.drug |
| Zdroj: | Immunology and Cell Biology. 70:173-179 |
| ISSN: | 0818-9641 |
| DOI: | 10.1038/icb.1992.22 |
| Popis: | Technetium-99 m (99mTc)-labelled conjugates of an anti-fibrin monoclonal antibody, DD-3B6/22, have been assessed for their detection of vascular thrombi in a rabbit model. DD-3B6/22 binds to a D-dimer epitope present on cross-linked fibrin but absent from the fibrin monomer or fibrinogen. Injection of a 99mTc-labelled Fab' fragment of DD-3B6/22 allowed delineation of model thrombi as early as 30 min postinjection (p.i.) with optimal localization at 4-5 h. Thrombus label uptake at 4 h p.i. was 0.304 +/- 0.106% injected dose/g (% ID/g) compared with 0.022 +/- 0.001% ID/g after the injection of a control Fab' fragment. These results suggest that the 99mTc-labelled Fab' fragment of DD-3B6/22 has excellent potential for scintigraphic detection of vascular thrombi in humans. |
| Databáze: | OpenAIRE |
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