5-HMF induces anaphylactoid reactions in vivo and in vitro
| Autor: | Sheng Lin, Encan Li, Guang Hu, Xiao-Yu Fan, Ruirui Hao, Qingfen Zhu, Lin Ni, Lin Lin, Hong-Tao Jin, Jiuming He |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Health
Toxicology and Mutagenesis Syk 010501 environmental sciences Pharmacology Toxicology 01 natural sciences Filamentous actin 5-HMF In vitro and in vivo animal models 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Western blot LYN In vivo lcsh:RA1190-1270 medicine Immunotoxicity 0105 earth and related environmental sciences lcsh:Toxicology. Poisons medicine.diagnostic_test Degranulation Anaphylactoid reaction In vitro chemistry 030217 neurology & neurosurgery Histamine |
| Zdroj: | Toxicology Reports, Vol 7, Iss, Pp 1402-1411 (2020) |
| ISSN: | 2214-7500 |
| Popis: | Aim Excessive exposure to 5-hydroxymethylfurfural (5-HMF), which is a common impurity in various sugar-containing products, induces serious side effects. Our previous study revealed that 5-HMF exerted immune sensitizing potential when injected into rodents. In this study, we explored 5-HMF mediated anaphylactoid reactions and its underlying molecular mechanisms. Methods We investigated anaphylactoid reactions in Brown Norway (BN) rats and Institute of Cancer Research (ICR) mice to identify 5-HMF mediated in vivo anaphylactoid reactions. RBL-2H3 and P815 cell degranulation models were also established, and degranulation, enzyme-linked immunosorbent, filamentous actin (F-actin) microfilament staining, and western blot assays were performed in these cells. Results We showed that 5-HMF induced anaphylactoid reactions by increasing blood vessel permeability in mice, and significantly elevating histamine (His) and glutathione peroxidase-1 (Gpx-1) levels in rat serum. Moreover, after incubation with 5-HMF, β-hexosaminidase (β-Hex), His, IL-4 and IL-6 levels were all significantly increased, thereby inducing cellular degranulation in RBL-2H3 and P815 cells. Finally, 5-HMF also upregulated Lyn, Syk, p38 and JNK protein phosphorylation levels. Conclusions Our findings suggest that 5-HMF induces anaphylactoid reactions both in vivo and in vitro, therefore 5-HMF limits in sugar-containing products should receive more regulatory attention. |
| Databáze: | OpenAIRE |
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