In Vivo Echocardiographic Imaging of Transplanted Human Adult Stem Cells in the Myocardium Labeled with Clinically Applicable CliniMACS Nanoparticles
| Autor: | M Makoui, Gregor Warnecke, Emmeran Gams, A Ghodsizad, HM Klein, Axel Haverich, Matthias Karck, Arjang Ruhparwar, Ulrich Martin, Michael Niehaus, Christoph Piechaczek, Christoph Bara |
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| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty Swine Cell Myocardial Infarction Contrast Media Human bone Cell Count In vivo medicine Animals Humans Distribution (pharmacology) Radiology Nuclear Medicine and imaging Cells Cultured Peripheral Blood Stem Cell Transplantation Immunomagnetic Separation business.industry Myocardium Stem Cells Image Enhancement medicine.disease Nanostructures Transplantation medicine.anatomical_structure Echocardiography Magnetic bead Heart failure Feasibility Studies Cardiology and Cardiovascular Medicine business human activities Echocardiography Transesophageal Adult stem cell |
| Zdroj: | Journal of the American Society of Echocardiography. 19:563-568 |
| ISSN: | 0894-7317 |
| Popis: | Objectives Intramyocardial transplantation of bone marrow–derived cells is currently under clinical evaluation as a therapy of heart failure. A major limitation of all clinical studies dealing with myocardial cell engraftment is the inability to track the fate of the transplanted cells. We present a clinically applicable technique using transesophageal echocardiography (TEE) of CliniMACS nanoparticle labeled transplanted CD133 + cells in ischemic hearts. Methods and Results CD133 + cells were isolated from human bone marrow by CliniMACS magnetic bead selection. Positive (5 × 10 6 cells) cells were transplanted into porcine ischemic myocardium (n = 6). Control animals (n = 5) received medium injection. TEE was performed to demonstrate the distribution of the cells during and 8 weeks after the procedure. Accumulations of labeled CD133 + cells of adjacent injections in the myocardium were discriminatively identified by TEE. Conclusions TEE is an elegant method for real-time documentation of successful transplantation and cell colony localization of magnetically labeled CD133 + cells in the ischemic myocardium. Our method should be of special interest for TEE-guided transcatheter injection of cells. |
| Databáze: | OpenAIRE |
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