Changes in brain cholinergic markers and spatial learning in old galanin-overexpressing mice
| Autor: | Alessandro Massella, Luciana Giardino, G. D’Intino, Marco Gusciglio, Sven-Ove Ögren, Tomas Hökfelt, Laura Calzà, Stefania Pirondi, Eugenia Kuteeva |
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| Přispěvatelé: | S. Pirondi, G. D'Intino, M. Gusciglio, A. Massella, L. Giardino, E. Kuteeva, S.O. Ogren, T. Hokfelt, L. Calza, Pirondi S., D’Intino G., Gusciglio M., Massella A., Giardino L., Kuteeva E., Ögren S.-O., Hökfelt T., Calzà L. |
| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Aging Neuropeptide Morris water navigation task Galanin Mice Transgenic Water maze Biology Motor Activity Hippocampus Choline O-Acetyltransferase Mice Prosencephalon Internal medicine medicine Animals Nerve Growth Factors RNA Messenger Cholinergic neuron Maze Learning Molecular Biology Swimming Cerebral Cortex Basal forebrain Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Brain-Derived Neurotrophic Factor Brain Choline acetyltransferase Immunohistochemistry Endocrinology nervous system Cholinergic Neurology (clinical) Biomarkers Developmental Biology |
| Zdroj: | Brain research. 1138 |
| ISSN: | 0006-8993 |
| Popis: | The cholinergic forebrain system is involved in learning and memory, and its age-dependent decline correlates with a decrease in cognitive performance. Since the neuropeptide galanin participates in cholinergic neuron regulation, we have studied 19- to 23-month-old male mice overexpressing galanin under the platelet-derived growth factor B promoter (GalOE) and wild-type (WT) littermates by monitoring behavioral, neurochemical and morphological/histochemical parameters. In the Morris water maze test, old transgenic animals showed a significant impairment in escape latency in the hidden platform test compared to age-matched WT animals. The morphological/histochemical studies revealed that cholinergic neurons in the basal forebrain display a slight, age- but not genotype-related, alteration in choline acetyltransferase- (ChAT) immunoreactivity. The neurochemical studies showed an age-related decline in ChAT activity in the cerebral cortex of all mice, whereas in the hippocampal formation this effect was seen in GalOE but not WT animals. Expression of BDNF mRNA in the hippocampal formation, as evaluated by RT-PCR, was reduced in old animals; no age- or genotype-induced variations in NGF mRNA expression were observed. These data suggest that galanin overexpression further accentuates the age-related decline of the cholinergic system activity in male mice, resulting in impairment of water maze performance in old animals. |
| Databáze: | OpenAIRE |
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