Expression pattern of four storage xyloglucan mobilization-related genes during seedling development of the rain forest tree Hymenaea courbaril L
| Autor: | Michel Georges Albert Vincentz, L. E. V. Del Bem, Marcos Silveira Buckeridge, A. D. Brandão |
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| Rok vydání: | 2009 |
| Předmět: |
DNA
Complementary Light Physiology Rain XTH β-galactosidase Plant Science Genes Plant invertase Trees Hypocotyl Cell wall chemistry.chemical_compound storage mobilization xyloglucan Gene Expression Regulation Plant Auxin Botany Hymenaea courbaril Glucans Phylogeny chemistry.chemical_classification Indoleacetic Acids beta-Fructofuranosidase biology fungi Glycosyltransferases food and beverages Biological Transport beta-Galactosidase biology.organism_classification Research Papers Actins Xyloglucan Invertase chemistry Glucosyltransferases Seedlings Seedling biology.protein cell wall Sucrose synthase Xylans Hymenaea PAREDE CELULAR VEGETAL |
| Zdroj: | Journal of Experimental Botany Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/erp014 |
| Popis: | During seedling establishment, cotyledons of the rain forest tree Hymenaea courbaril mobilize storage cell wall xyloglucan to sustain growth. The polysaccharide is degraded and its products are transported to growing sink tissues. Auxin from the shoot controls the level of xyloglucan hydrolytic enzymes. It is not yet known how important the expression of these genes is for the control of storage xyloglucan degradation. In this work, partial cDNAs of the genes xyloglucan transglycosylase hydrolase (HcXTH1) and beta-galactosidase (HcBGAL1), both related to xyloglucan degradation, and two other genes related to sucrose metabolism [alkaline invertase (HcAlkIN1) and sucrose synthase (HcSUS1)], were isolated. The partial sequences were characterized by comparison with sequences available in the literature, and phylogenetic trees were assembled. Gene expression was evaluated at intervals of 6 h during 24 h in cotyledons, hypocotyl, roots, and leaves, using 45-d-old plantlets. HcXTH1 and HcBGAL1 were correlated to xyloglucan degradation and responded to auxin and light, being down-regulated when transport of auxin was prevented by N-1-naphthylphthalamic acid (NPA) and stimulated by constant light. Genes related to sucrose metabolism, HcAlkIN1 and HcSUS1, responded to inhibition of auxin transport in consonance with storage mobilization in the cotyledons. A model is proposed suggesting that auxin and light are involved in the control of the expression of genes related to storage xyloglucan mobilization in seedlings of H. courbaril. It is concluded that gene expression plays a role in the control of the intercommunication system of the source-sink relationship during seeding growth, favouring its establishment in the shaded environment of the rain forest understorey. |
| Databáze: | OpenAIRE |
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