Ex vivo and in vivo studies of Viola tricolor Linn. as potential cardio protective and hypotensive agent: Inhibition of voltage‐gated Ca ++ ion channels
| Autor: | Marius Moga, Luigi Geo Marceanu, Muhammad Arif Aslam, Alotaibi O. Modhi, Elena Bobescu, Khizra Mujahid, Fatima Saqib |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Cardiotonic Agents medicine.drug_class Vasodilator Agents Myocardial Infarction Vasodilation Calcium channel blocker Pharmacology Biochemistry Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Viola In vivo Renin–angiotensin system Genetics medicine Animals cardiovascular diseases Rats Wistar Molecular Biology Phenylephrine Cyclic guanosine monophosphate Plant Extracts Isoproterenol Rats 030104 developmental biology chemistry cardiovascular system Calcium Channels Rabbits Hypotension 030217 neurology & neurosurgery Ex vivo Biotechnology medicine.drug |
| Zdroj: | The FASEB Journal. 34:9102-9119 |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fj.202000658r |
| Popis: | Viola tricolor Linn. is used as cardio-protective and anti-hypertensive agent in traditional medicine. Current study objective was to evaluate cardio-protective and hypotensive effects of Viola tricolor L. in vitro and in vivo studies. Viola tricolor L. crude extract (Vt.Cr) and its fractions (Aqueous and organic) were tested at rabbit atria and aorta coupled to Power Lab Data Acquisition System for cardio depressant and vasorelaxant effects in vitro whereas in vivo Blood Pressure was checked by invasive method in normotensive ketamine-diazepam anesthetized rats. Isoproterenol was employed for acute myocardial infarction (AMI) and left ventricular hypertrophy (LVH) development and cardioprotective effects of Vt.Cr were evaluated hemodynamically and histopathologically. Vt.Cr and its fractions decreased heart rate and contractile force in paired atria and relaxed Phenylephrine (1 µM) and K+ (80 mM) stimulated contractions in aorta possibly mediated through Voltage dependent L-type calcium channels blockage supported by in vivo hypotensive action. In LVH, Vt.Cr lowered Angiotensin Converting Enzymes and renin, increased cyclic Guanosine Monophosphate and nitric oxide levels, decreased cardiomyocytes size and fibrosis attributed to Gallic acid as detected by High Performance Liquid Chromatography. Partial positive results were seen hemodynamically and histologically in AMI Viola tricolor L. showed vasorelaxant, cardio-relaxant, hypotensive, and cardio protective effect validating traditional practice in cardiovascular disorders. |
| Databáze: | OpenAIRE |
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