| Autor: |
Edward D. DeLamater, M. Schaechter, E.L. Treece |
| Rok vydání: |
1954 |
| Předmět: |
|
| Zdroj: |
Experimental Cell Research. 6:361-366 |
| ISSN: |
0014-4827 |
| DOI: |
10.1016/0014-4827(54)90184-6 |
| Popis: |
The Gomori staining reaction for alkaline phosphatase was carried out in several bacteria. Fixation with acetone did not interfere with the staining reaction, whereas pretreatment with acid citrate buffer proved inhibitory to the enzyme. The optimum pH zone of the enzyme in B. subtilis (pH 8.8–9.2) was found to be close to that at which the staining reaction was carried out (pH 9.4). The intensity of staining was proportional to the concentration of calcium in the incubating mixture. No concomitant change in localization was found. The addition of manganese to the culture medium significantly increased the staining intensity in ten species. The localization of the stain in seven species was in cytoplasmic granules which appeared to correspond to those described as mitochondria by previous authors. Staining of cell walls and of the entire cytoplasm was also found. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
