One-step nucleic acid amplification testing in medullary thyroid cancer lymph nodes: a case series
| Autor: | Wojciech Młynarski, Anna Grzegory, Maciej Borowiec, Katarzyna Paduszyńska, Wojciech Fendler, Lech Pomorski, Krzysztof Kaczka |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty business.industry Medullary thyroid cancer General Medicine medicine.disease medullary thyroid cancer Metastasis medicine.anatomical_structure one-step nucleic acid amplification Clinical Research lymph nodes Cervical lymph nodes histopathology medicine Nucleic acid Immunohistochemistry Histopathology Lymph business Lymph node |
| Zdroj: | Archives of Medical Science : AMS |
| ISSN: | 1734-1922 |
| DOI: | 10.5114/aoms.2015.49206 |
| Popis: | INTRODUCTION: Locoregional relapse in medullary thyroid cancer (MTC) may be caused by nodal micrometastases. Medullary thyroid cancer lymph nodes have not yet been evaluated by one-step nucleic acid amplification (OSNA). Therefore, the aim of this study was to detect MTC cells by OSNA in cervical lymph nodes and compare the obtained outcomes with conventional histopathology. MATERIAL AND METHODS: Twenty-one randomized, unenlarged lymph nodes from 5 patients with MTC were examined by histopathology and OSNA. Lymph nodes were divided into four representative blocks by a sterile, single use, special cutting device in the same way as in the clinical protocol study performed by Tsujimoto et al. Two blocks were used for histopathology and immunohistochemistry, 2 for OSNA. RESULTS: Positive results of histopathology and OSNA were revealed in 4 patients. The outcomes of OSNA and histopathology were corresponding in 3 patients. Positive histopathology results of 2 lymph nodes from 2 patients were confirmed by OSNA. In 1 patient there were only negative results of both examinations. One-step nucleic acid amplification failed to detect metastasis in 1 lymph node in 2 patients although it did not change the TNM status in these patients. There were no false positive results in the OSNA test. CONCLUSIONS: One-step nucleic acid amplification may be an alternative method to histopathology in detecting nodal involvement in MTC. Further studies should evaluate the sensitivity and specificity of OSNA and the impact on staging in MTC. |
| Databáze: | OpenAIRE |
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