Endocrine-disruption potential of perfluoroethylcyclohexane sulfonate (PFECHS) in chronically exposed Daphnia magna
| Autor: | Mélanie Lépine, Keven Jean, Mélanie Douville, Maeva Giraudo, Amila O. De Silva, Magali Houde, Christine Spencer |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Aquatic Organisms Canada Microarray Health Toxicology and Mutagenesis Population Daphnia magna 010501 environmental sciences Biology Endocrine Disruptors Toxicology 01 natural sciences Transcriptome Biological pathway 03 medical and health sciences chemistry.chemical_compound Vitellogenins Rivers Tandem Mass Spectrometry Animals Amino Acid Sequence education 0105 earth and related environmental sciences education.field_of_study Fluorocarbons Reproduction Aquatic animal General Medicine biology.organism_classification Pollution Molecular biology Up-Regulation Perfluorooctane 030104 developmental biology chemistry Alkanesulfonic Acids Daphnia Environmental chemistry Toxicity Sulfonic Acids Biomarkers Water Pollutants Chemical |
| Zdroj: | Environmental pollution (Barking, Essex : 1987). 218 |
| ISSN: | 1873-6424 |
| Popis: | Perfluoroethylcyclohexane sulfonate (PFECHS), mainly used in hydraulic fluids in aircrafts, is a member of the perfluoroalkyl sulfonate family which includes the regulated perfluorooctane sulfonate (PFOS). PFECHS has been reported in environmental samples but its toxicity to aquatic organisms is unknown. The objectives of this study were to identify biological pathways altered by sublethal exposure (12 d) of D. magna to PFECHS (0.06, 0.6, and 6 mg/L) using microarray and quantitative real-time PCR and to identify potential biomarkers to link transcriptomic to phenotypic responses. PFECHS was also quantified in surface water samples (1.04–1.38 ng/L) collected from the St. Lawrence River, Canada. Transcriptomic analyses indicated the under-regulation of vitellogenin-related genes ( VTG1 ) in PFECHS-exposed groups. PFECHS exposure also led to the up-regulation of genes related to cuticle. VTG was selected as a potential cellular marker and identified in D. magna using an immuno-specific assay and quantified using Western blot and LC/MS/MS. Results indicated a decrease of VTG content in exposed D. magna which was in concordance with the transcription of VTG-related genes. No effects were observed on survival, molting, or reproduction at the individual/population levels. Overall, results suggest endocrine disruption potential for PFECHS in D. magna at concentrations higher than levels reported in the aquatic environment. |
| Databáze: | OpenAIRE |
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