Brainstem prolactin-releasing peptide contributes to cancer anorexia-cachexia syndrome in rats
| Autor: | Keila Navarro I Batista, Marissa Schraner, Thomas Riediger |
|---|---|
| Přispěvatelé: | University of Zurich, Riediger, Thomas |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Cachexia Carcinoma Hepatocellular Prolactin-releasing peptide 2804 Cellular and Molecular Neuroscience Neuropeptide Myostatin Energy homeostasis 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Cell Line Tumor Internal medicine medicine Animals Rats Inbred BUF PI3K/AKT/mTOR pathway Pharmacology Prolactin-Releasing Hormone Gene knockdown biology business.industry Liver Neoplasms Area postrema 10081 Institute of Veterinary Physiology medicine.disease Anorexia Rats 3004 Pharmacology 030104 developmental biology Endocrinology Gene Knockdown Techniques biology.protein 570 Life sciences business 030217 neurology & neurosurgery Brain Stem |
| Zdroj: | Neuropharmacology. 180:108289 |
| ISSN: | 0028-3908 |
| Popis: | Up to 80% of cancer patients are affected by the cancer anorexia-cachexia syndrome (CACS), which leads to excessive body weight loss, reduced treatment success and increased lethality. The area postrema/nucleus of the solitary tract (AP/NTS) region emerged as a central nervous key structure in this multi-factorial process. Neurons in this area are targeted by cytokines and signal to downstream sites involved in energy homeostasis. NTS neurons expressing prolactin-releasing peptide (PrRP) are implicated in the control of energy intake and hypothalamus-pituitary-adrenal (HPA) axis activation, which contributes to muscle wasting. To explore if brainstem PrRP neurons contribute to CACS, we selectively knocked down PrRP expression in the NTS of hepatoma tumor-bearing rats by an AAV/shRNA gene silencing approach. PrRP knockdown reduced body weight loss and anorexia compared to tumor-bearing controls treated with a non-silencing AAV. Gastrocnemius and total hind limb muscle weight was higher in PrPR knockdown rats. Corticosterone levels were increased in the early phase after tumor induction at day 6 in both groups but returned to baseline levels at day 21 in the PrRP knockdown group. While we did not detect significant changes in gene expression of markers for muscle protein metabolism (MuRF-1, myostatin, mTOR and REDD1), mTOR and REDD1 tended to be lower after disruption PrRP signalling. In conclusion, we identified brainstem PrRP as a possible neuropeptide mediator of CACS in hepatoma tumor-bearing rats. The central and peripheral downstream mechanisms require further investigation and might involve HPA axis activation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect