Trait analysis reveals DOG1 determines initial depth of seed dormancy, but not changes during dormancy cycling that result in seedling emergence timing
| Autor: | Steven Penfield, William E. Finch-Savage, Angela J. Hambidge, Steven Footitt, James R. Lynn, Peter Glen Walley |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine QTL analysis Physiology Population Arabidopsis Germination Plant Science Quantitative trait locus Biology 01 natural sciences Cape verde 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant dormancy cycling Botany ABA signalling education Abscisic acid education.field_of_study Full Paper Ecotype Arabidopsis Proteins Research QH QK seed dormancy Seed dormancy food and beverages Full Papers DOG1 biology.organism_classification Plant Dormancy 030104 developmental biology chemistry Seedling Seedlings Seeds Dormancy Seasons Abscisic Acid 010606 plant biology & botany Transcription Factors |
| Zdroj: | The New phytologist The New Phytologist |
| ISSN: | 1469-8137 0028-646X |
| Popis: | Summary Seedling emergence timing is crucial in competitive plant communities and so contributes to species fitness. To understand the mechanistic basis of variation in seedling emergence timing, we exploited the contrasting behaviour of two Arabidopsis thaliana ecotypes: Cape Verde Islands (Cvi) and Burren (Bur‐0).We used RNA‐Seq analysis of RNA from exhumed seeds and quantitative trait loci (QTL) analyses on a mapping population from crossing the Cvi and Bur‐0 ecotypes.We determined genome‐wide expression patterns over an annual dormancy cycle in both ecotypes, identifying nine major clusters based on the seasonal timing of gene expression, and variation in behaviour between them. QTL were identified for depth of seed dormancy and seedling emergence timing (SET).Both analyses showed a key role for DOG1 in determining depth of dormancy, but did not support a direct role for DOG1 in generating altered seasonal patterns of seedling emergence. The principle QTL determining SET (SET1: dormancy cycling) is physically close on chromosome 5, but is distinct from DOG1. We show that SET1 and two other SET QTLs each contain a candidate gene (AHG1, ANAC060, PDF1 respectively) closely associated with DOG1 and abscisic acid signalling and suggest a model for the control of SET in the field. See also the Commentary on this article by https://doi.org/10.1111/nph.16254. |
| Databáze: | OpenAIRE |
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