Glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase genes of winter wheat enhance the cold tolerance of transgenic Arabidopsis
| Autor: | Yu Tian, Da Zhang, Jing Meng, Yuzhuo Bao, Duojia Wang, Kankan Peng, Jing Cang, Xiaonan Wang |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Transgene Arabidopsis Plant Science Glucosephosphate Dehydrogenase Pentose phosphate pathway 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Stress Physiological Genetics Glucose-6-phosphate dehydrogenase Abscisic acid Triticum Plant Proteins chemistry.chemical_classification Reactive oxygen species biology Abiotic stress Phosphogluconate Dehydrogenase food and beverages Peroxisome Plants Genetically Modified biology.organism_classification Cold Temperature 030104 developmental biology chemistry Biochemistry Abscisic Acid 010606 plant biology & botany |
| Zdroj: | Plant Physiology and Biochemistry. 161:86-97 |
| ISSN: | 0981-9428 |
| Popis: | In this study, winter wheat G6PDH (TaG6PDH) and 6PGDH (Ta6PGDH) were investigated. Both their expression and their activity were upregulated under cold stress, suggesting that TaG6PDH and Ta6PGDH positively respond to cold stress in winter wheat. Exogenous abscisic acid (ABA) treatment markedly increased the expression and activity levels of TaG6PDH and Ta6PGDH in winter wheat under cold stress. Subsequently, TaG6PDH-and Ta6PGDH were overexpressed in Arabidopsis, and showed stronger reactive oxygen species (ROS)-scavenging ability and higher survival rate compared with wild-type (WT) plants under cold stress. In addition, we found that TaG6PDH and Ta6PGDH overexpression can promote the oxidative pentose phosphate pathway (OPPP) in the cytoplasm and peroxisomes of Arabidopsis. In summary, Arabidopsis overexpressing TaG6PDH and Ta6PGDH showed improved cold tolerance. |
| Databáze: | OpenAIRE |
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