Acridine Orange Excited by Low-Dose Radiation Has a Strong Cytocidal Effect on Mouse Osteosarcoma
Autor: | Mitsuoki Hashiba, Katsuyuki Kusuzaki, Shin Hashiguchi, Hiroaki Murata, Yasusuke Hirasawa, Tsukasa Ashihara, Tsunehiko Nishimura, Hideyuki Takeshita |
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Rok vydání: | 2002 |
Předmět: |
Cancer Research
Lung Neoplasms Time Factors Combination therapy Cell Survival medicine.medical_treatment Photodynamic therapy Mice chemistry.chemical_compound In vivo Tumor Cells Cultured medicine Animals Photosensitizer Cell Size Osteosarcoma Chemistry business.industry X-Rays Acridine orange Dose-Response Relationship Radiation General Medicine medicine.disease Acridine Orange In vitro Survival Rate Radiation therapy Microscopy Fluorescence Photochemotherapy Oncology Cancer research Nuclear medicine business Neoplasm Transplantation |
Zdroj: | Oncology. 62:85-93 |
ISSN: | 1423-0232 0030-2414 |
Popis: | The study was conducted to clarify the cytocidal effect of combination therapy consisting of administration of acridine orange (AO), which is a photosensitizer, and radiation therapy using in vitro and in vivo mouse osteosarcoma models. The results revealed that AO combined with low-dose X-ray irradiation of about 1–5 Gy had a strong cytocidal effect on the cultured mouse osteosarcoma cells regardless of their chemosensitivity, and that this combination therapy inhibited growth of the in vivo mouse osteosarcoma by induction of tumor necrosis. This effect was inhibited by L-histidine, but not by mannitol. These findings suggested that AO might be excited by X-rays and kill osteosarcoma cells through the release of singlet oxygen, which is toxic to living cells. This mechanism is similar to that of photodynamic therapy with AO. |
Databáze: | OpenAIRE |
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