Acridine Orange Excited by Low-Dose Radiation Has a Strong Cytocidal Effect on Mouse Osteosarcoma

Autor: Mitsuoki Hashiba, Katsuyuki Kusuzaki, Shin Hashiguchi, Hiroaki Murata, Yasusuke Hirasawa, Tsukasa Ashihara, Tsunehiko Nishimura, Hideyuki Takeshita
Rok vydání: 2002
Předmět:
Zdroj: Oncology. 62:85-93
ISSN: 1423-0232
0030-2414
Popis: The study was conducted to clarify the cytocidal effect of combination therapy consisting of administration of acridine orange (AO), which is a photosensitizer, and radiation therapy using in vitro and in vivo mouse osteosarcoma models. The results revealed that AO combined with low-dose X-ray irradiation of about 1–5 Gy had a strong cytocidal effect on the cultured mouse osteosarcoma cells regardless of their chemosensitivity, and that this combination therapy inhibited growth of the in vivo mouse osteosarcoma by induction of tumor necrosis. This effect was inhibited by L-histidine, but not by mannitol. These findings suggested that AO might be excited by X-rays and kill osteosarcoma cells through the release of singlet oxygen, which is toxic to living cells. This mechanism is similar to that of photodynamic therapy with AO.
Databáze: OpenAIRE