Near infrared fluorescence imaging of rabbit thyroid and parathyroid glands
| Autor: | Barney Harrison, Nicola J. Brown, R. Antakia, Tim Stephenson, Pascal Gayet, Saba P. Balasubramanian, Stephanie Guillermet |
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| Rok vydání: | 2014 |
| Předmět: |
Male
endocrine system Near-Infrared Fluorescence Imaging Pathology medicine.medical_specialty Thyroid Gland Parathyroid Glands chemistry.chemical_compound Intraoperative Period Medicine Animals Enzyme Inhibitors Vein Spectroscopy Near-Infrared business.industry Dissection Thyroid Washout Histology Fluorescence Methylene Blue medicine.anatomical_structure Spectrometry Fluorescence chemistry Injections Intravenous Surgery Parathyroid gland Female Rabbits business Methylene blue Neck |
| Zdroj: | The Journal of surgical research. 192(2) |
| ISSN: | 1095-8673 |
| Popis: | Background Near infrared fluorescence imaging using intravenous methylene blue (MB) is a novel technique that has potential to aid the parathyroid gland (PG) localization during thyroid and parathyroid surgery. The aim of this study was to examine MB fluorescence in the rabbit neck and determine the influence of MB dose and time following administration on fluorescence from thyroid and PGs. Methods Thyroid and external PGs were exposed in six New Zealand white rabbits under anesthesia. Varying doses of MB (0.025–3 mg/kg) were injected through the marginal ear vein. Near infrared fluorescence from exposed tissues was recorded at different time intervals (10–74 min) using Fluobeam 700. Specimens of identified glands were then resected for histologic assessment. Results Histology confirmed accurate identification of all excised thyroid and PGs; these were the only neck structures to demonstrate significant fluorescence. The parathyroid demonstrated lower fluorescence intensities and reduced washout times at all MB doses compared with the thyroid gland. A dose of 0.1 mg/kg MB was adequate to identify fluorescence; this also delineated the blood supply of the external PGs. Conclusions The study demonstrates that near infrared fluorescence with intravenous MB helps differentiate between thyroid and PGs in the rabbit. This has potential to improve outcomes in thyroid and parathyroid surgery by increasing the accuracy of parathyroid identification; however, the findings require replication in human surgery. The use of low doses of MB may also avoid the side effects associated with currently used doses in humans (3–7 mg/kg). |
| Databáze: | OpenAIRE |
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