C-C motif ligand 5 promotes migration of prostate cancer cells in the prostate cancer bone metastasis microenvironment
| Autor: | Suguru Kadomoto, Tomoyuki Makino, Aerken Maolake, Kazutaka Narimoto, Kazuyoshi Shigehara, Wen-Jye Lin, Guzailinuer Wufuer, Atsushi Mizokami, Ariunbold Natsagdorj, Renato Naito, Hiroaki Iwamoto, Yoshifumi Kadono, Kouji Izumi, Satoko Urata, Kaoru Hiratsuka |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Cancer Research Stromal cell Protein Array Analysis bone stromal cells Bone Neoplasms migration urologic and male genital diseases 03 medical and health sciences Prostate cancer 0302 clinical medicine Cell Molecular and Stem Cell Biology Cell Movement androgen receptor Cell Line Tumor LNCaP Tumor Microenvironment medicine Humans Chemokine CCL5 C‐C motif ligand 5 Cell Proliferation Tumor microenvironment Chemistry Prostatic Neoplasms Bone metastasis Cell migration Original Articles General Medicine prostate cancer medicine.disease Chemokine activity Coculture Techniques Up-Regulation Gene Expression Regulation Neoplastic 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer cell Cancer research Original Article Stromal Cells |
| Zdroj: | Cancer Science |
| ISSN: | 1347-9032 |
| DOI: | 10.1111/cas.13494 |
| Popis: | Chemokines and their receptors have key roles in cancer progression. The present study investigated chemokine activity in the prostate cancer bone metastasis microenvironment. Growth and migration of human prostate cancer cells were assayed in cocultures with bone stromal cells. The migration of LNCaP cells significantly increased when co-cultured with bone stromal cells isolated from prostate cancer bone metastases. Cytokine array analysis of conditioned medium from bone stromal cell cultures identified CCL5 as a concentration-dependent promoter of LNCaP cell migration. The migration of LNCaP cells was suppressed when C-C motif ligand 5 (CCL5) neutralizing antibody was added to cocultures with bone stromal cells. Knockdown of androgen receptor with small interfering RNA increased the migration of LNCaP cells compared with control cells, and CCL5 did not promote the migration of androgen receptor knockdown LNCaP. Elevated CCL5 secretion in bone stromal cells from metastatic lesions induced prostate cancer cell migration by a mechanism consistent with CCL5 activity upstream of androgen receptor signaling. |
| Databáze: | OpenAIRE |
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