Effective role of medium supplementation in microalgal lipid accumulation
| Autor: | Sirous Ebrahimi, Azadeh Fazeli Danesh, Mark C.M. van Loosdrecht, Peter R. Mooij, Robbert Kleerebezem |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Vitamin Lipid accumulation Nitrogen Starch chemistry.chemical_element Bioengineering 01 natural sciences Applied Microbiology and Biotechnology Enrichment culture 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Microalgae Food science Starch formation Phosphorus Lipid Metabolism Culture Media 030104 developmental biology chemistry Lipid content Biodiesel production Carbohydrate Metabolism Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 115:1152-1160 |
| ISSN: | 0006-3592 |
| DOI: | 10.1002/bit.26548 |
| Popis: | The present study investigated the interaction between starch and lipid accumulation in a green microalgae enrichment culture. The objective was to optimize the lipid content by manipulation of the medium in regular batch culture. Two medium designs were evaluated: First a high ortho-P concentration with vitamin supplementary (Pi-vitamins supplemented medium), second normal growth medium (control). Both media contained a low amount of nitrogen which was consumed during batch growth in three days. The batch experiments continued for another 4 days with the absence of soluble nitrogen in the medium. When the mixed microalgal culture was incubated in the Pi-vitamin supplemented medium, the lipid, and starch content of the culture increased within the first 3 days to 102.0 ± 5.2 mg/L (12.7 ± 0.6% of DW) and 31.7 ± 1.6 mg/L (4.0 ± 0.2% of DW), respectively. On the last day of the experiment, the lipid, and starch content in Pi-vitamin medium increased to 663.1 ± 32.5 mg/L (33.4 ± 1.6% of DW) and 127.5 ± 5.2 mg/L (6.4 ± 0.3% of DW). However, the lipid and starch content in the control process, reached to 334.7 ± 16.4 mg/L (20.1 ± 1.0% of DW) and 94.3 ± 4.6 mg/L (5.7 ± 0.3% of DW), respectively. The high Pi-vitamin medium induced storing lipid formation clearly while the starch formation was not affected. The lipid contents reported here are among the high reported in the literature, note that already under full growth conditions significant lipid levels occurred in the algal enrichment culture. The high lipid productivity of the reported mixed microalgae culture provides an efficient route for efficient algal biodiesel production. |
| Databáze: | OpenAIRE |
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