Molecular cloning of APRF, a novel IFN-stimulated gene factor 3 p91-related transcription factor involved in the gp130-mediated signaling pathway
| Autor: | Shizuo Akira, Xue-Jie Wang, Yukihiro Nishio, Taiji Matsusaka, Masanobu Naruto, Masahiro Inoue, Tadamitsu Kishimoto, Tetsuo Sudo, Shi We, Kanji Yoshida |
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| Rok vydání: | 1994 |
| Předmět: |
STAT3 Transcription Factor
DNA Complementary Molecular Sequence Data Response element Nerve Tissue Proteins Oncostatin M Biology Leukemia Inhibitory Factor General Biochemistry Genetics and Molecular Biology Mice chemistry.chemical_compound Animals Humans Tissue Distribution Amino Acid Sequence Ciliary Neurotrophic Factor Cloning Molecular Phosphotyrosine Transcription factor Lymphokines Base Sequence Sequence Homology Amino Acid Interleukin-6 Tyrosine phosphorylation Interferon-Stimulated Gene Factor 3 Glycoprotein 130 Molecular biology Growth Inhibitors Interferon-Stimulated Gene Factor 3 gamma Subunit DNA-Binding Proteins Oligodeoxyribonucleotides chemistry Trans-Activators Tyrosine Phosphorylation Signal transduction Peptides Sequence Alignment Signal Transduction Transcription Factors |
| Zdroj: | Cell. 77:63-71 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/0092-8674(94)90235-6 |
| Popis: | Acute-phase response factor (APRF) is a transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. We report here the purification and cloning of APRF. APRF exhibits a 52.5% overall homology at the amino acid level with p91, a component of the interferon (IFN)-stimulated gene factor 3 complexes. The cloned APRF protein is tyrosine phosphorylated and translocated into the nucleus in response to IL-6, but not in response to IFN-γ. Tyrosine phosphorylation was also observed in response to other cytokines, such as leukemia inhibitory factor, oncostatin M, and ciliary neurotrophic factor, whose receptors share the IL-6 receptor signal transducer gp130. In contrast, we observed that p91 is not tyrosine phosphorylated in response to IL-6. These results suggest that this novel p91-related protein may play a major role in the gp130-mediated signaling pathway and that selective activation of p91-related factors may explain the diversity of cellular responses to different cytokines. |
| Databáze: | OpenAIRE |
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