Squalene isolated from Schizochytrium mangrovei is a peroxisome proliferator-activated receptor-α agonist that regulates lipid metabolism in HepG2 cells
| Autor: | Thi Minh Hien Hoang, Diem Hong Dang, Thi Huong Quynh Hoang, Thi Lan Anh Hoang, Cam Ha Nguyen, Thi Hoai Thu Ngo, Thi Thom Le |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Squalene Peroxisome proliferator-activated receptor gamma Peroxisome proliferator-activated receptor Bioengineering Biology Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Microalgae Humans PPAR alpha Beta oxidation Fatty acid synthesis chemistry.chemical_classification 030109 nutrition & dietetics Fatty Acids Fatty acid Lipid metabolism General Medicine Hep G2 Cells Lipid Metabolism 030104 developmental biology Biochemistry chemistry Hepatocytes Peroxisome proliferator-activated receptor alpha Biotechnology |
| Zdroj: | Biotechnology letters. 38(7) |
| ISSN: | 1573-6776 |
| Popis: | Peroxisome proliferator-activated receptor-alpha (PPARα) is a nuclear receptor that has critical roles in the treatment of atherosclerosis, hyperlipidemia and hepatic steatosis. Squalene is a novel nature PPARα agonist, identified from reporter gene assay and qPCR analysis. Cultured hepatocytes stimulated with squalene exhibited significantly decreased cellular triacylglycerols and cholesterol concentrations, while cellular uptake of fatty acids was increased. Quantitative PCR analysis revealed that expression of genes related to fatty acid uptake, fatty acid oxidation, ketogenesis and reverse cholesterol transport metabolism were upregulated, while that of genes related to fatty acid synthesis were suppressed in cell treated with squalene. Squalene is hypolipidemic by activation of PPARα via a ligand-mediated mechanism that regulates the expression of lipid metabolism genes in hepatocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink