Human serum albumin attenuates global cerebral ischemia/reperfusion-induced brain injury in a Wnt/β-Catenin/ROS signaling-dependent manner in rats

Autor: Fei-Yu Yang, Ming Liu, Yuedong Tang, Feng Zhang, Jie Shen
Rok vydání: 2019
Předmět:
0301 basic medicine
medicine.medical_specialty
β-Catenin
Ischemia
RM1-950
Brain damage
Brain Ischemia
Wnt
Random Allocation
03 medical and health sciences
symbols.namesake
0302 clinical medicine
Western blot
Internal medicine
medicine
Animals
Humans
Serum Albumin
beta Catenin
Pharmacology
medicine.diagnostic_test
Chemistry
Wnt signaling pathway
Antagonist
Human serum albumin
Global cerebral ischemia
General Medicine
medicine.disease
Rats
Up-Regulation
Wnt Proteins
body regions
Oxidative Stress
Neuroprotective Agents
030104 developmental biology
Endocrinology
Gene Expression Regulation
Reperfusion Injury
030220 oncology & carcinogenesis
Catenin
embryonic structures
Nissl body
symbols
Immunohistochemistry
Therapeutics. Pharmacology
medicine.symptom
Reactive Oxygen Species
Signal Transduction
Zdroj: Biomedicine & Pharmacotherapy, Vol 115, Iss, Pp-(2019)
ISSN: 0753-3322
DOI: 10.1016/j.biopha.2019.108871
Popis: This study sought to clarify the role and underlying mechanisms of human serum albumin (HSA) therapy in global cerebral ischemia/reperfusion (GCI/R)-induced brain damage in rats. Five groups of adult male Wistar rats (n = 12 per group) were created as follows: sham operation (Sham), global cerebral ischemia/reperfusion (GCI/R), HSA treatment (GCI/R + HSA), Dickkopf-1 (DDK1) treatment (GCI/R + DDK1), and DDK1 plus HSA treatment (GCI/R + DKK1 + HSA). The GCI/R injury model was created using the modified Pusinelli four-vessel occlusion method. After 24 h, rats were evaluated using neurological scoring, Nissl staining, and brain tissue water content. The mRNA expression of Wnt, GSK3β, and β-Catenin in the brain were detected by quantitative real time polymerase chain reaction. The protein expression of β-Catenin and GSK-3β were investigated by western blot and immunohistochemical analysis in the presence and absence of the Wnt/β-Catenin antagonist, DKK-1. Complex I activity and ROS content were also measured. After 24 h of reperfusion, the behavior score and brain tissue water content in the GCI/R + HSA group were lower than that in the GCI/R group. In addition, the degree of neuronal injury was significantly reduced in the GCI/R + HSA group (P
Databáze: OpenAIRE
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