Chromosomal instability in haemopoietic cells of the foetus, mother and offspring after in utero irradiation of the CBA/Ca mouse
| Autor: | M. Rosemann, B. E. Lambert, A. Milner |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Genetics
Chromosome Aberrations Fetus Radiological and Ultrasound Technology Ratón CBA/Ca Mouse Offspring X-Rays Chromosome Bone Marrow Cells Biology Hematopoietic Stem Cells Plutonium Andrology Mice In utero Pregnancy Chromosome instability Mice Inbred CBA Animals Radiology Nuclear Medicine and imaging Female Stem cell |
| Zdroj: | International journal of radiation biology. 75(5) |
| ISSN: | 0955-3002 |
| Popis: | The present study was conducted to test the susceptibility of the mouse foetus to transmit chromosomal instability to the haemopoietic stem cells of offspring after in utero X-or plutonium-239-irradiation.Pregnant CBA/Ca-mice were injected with 80 kBq/kg 239Pu or X-irradiated with 1 Gy X-rays on days 13 or 14 of gestation. CFU-A cultures were grown from haemopoietic stem cells sampled from foetal liver and the bone marrow from the offspring and from the mother. Non-clonal, unstable chromosomal aberrations were scored in metaphases from individual stem cell colonies.The relative excess (RE) of unstable chromosomal aberrations in foetal liver cells irradiated with 1 Gy X-rays increased from 1.6 at day 2 up to 2.7 at day 4 after irradiation. In the bone marrow cells from the mother, this value was 1.8 (average from cells sampled at days 3 and 14 after irradiation). After injection of the pregnant mice with 235Pu, the yield of unstable chromosomal aberrations per cell was 0.14+/-0.03 (RE approximately 10) in descendants of bone marrow cells from the mother, 0.11+/-0.02 (RE = 10) in descendants of foetal liver cells and 0.16+/-0.05 (RE = 10) in descendants of bone marrow cells from the offspring.From the numerical analysis of non-clonal, unstable aberrations in haemopoietic cells from the foetus, the mother and the offspring after in utero irradiation, it was concluded that in utero irradiation of the CBA/Ca mouse was not more efficient in inducing chromosomal instability in the offspring than in the foetus or the mother. All three cell populations exhibited a similar degree of unstable aberrations, both in terms of the absolute numbers of non-clonal aberrations and in terms of relative excess compared with unexposed controls. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|