LPS-induced microvascular leukocytosis can be assessed by blue-field entoptic phenomenon
| Autor: | Elzbieta Polska, Julia Kolodjaschna, Michael Wolzt, Fatmire Berisha, Bernd Jilma, Georg Schaller, Solveig Lung, Leopold Schmetterer |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Lipopolysaccharides Male Pathology medicine.medical_specialty Erythrocytes Lipopolysaccharide Leukocytosis Physiology Inflammation Vasodilation Pharmacology Eye medicine.disease_cause Microcirculation Leukocyte Count chemistry.chemical_compound Cell Movement Physiology (medical) Laser-Doppler Flowmetry medicine Humans Escherichia coli business.industry Retinal Vessels Blue field entoptic phenomenon chemistry Circulatory system medicine.symptom Cardiology and Cardiovascular Medicine business Blood Flow Velocity |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 287:H691-H694 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.01240.2003 |
| Popis: | Administration of low doses of Escherichia coli endotoxin [a lipopolysaccharide (LPS)] to humans enables the study of inflammatory mechanisms. The purpose of the present study was to investigate whether the blue-field entoptic technique may be used to quantify the increase in circulating leukocytes in the ocular microvasculature after LPS infusion. In addition, combined laser Doppler velocimetry and retinal vessel size measurement were used to study red blood cell movement. Twelve healthy male volunteers received 20 IU/kg iv LPS as a bolus infusion. Outcome parameters were measured at baseline and 4 h after LPS administration. In the first protocol (n = 6 subjects), ocular hemodynamic effects were assessed with the blue-field entoptic technique, the retinal vessel analyzer, and laser Doppler velocimetry. In the second protocol (n = 6 subjects), white blood cell (WBC) counts from peripheral blood samples and blue-field entoptic technique measurements were performed. LPS caused peripheral blood leukocytosis and increased WBC density in ocular microvessels (by 49%; P = 0.036) but did not change WBC velocity. In addition, retinal venous diameter was increased (by 9%; P = 0.008), but red blood cell velocity remained unchanged. The LPS-induced changes in retinal WBC density and leukocyte counts were significantly correlated (r = 0.87). The present study indicates that the blue-field entoptic technique can be used to assess microvascular leukocyte recruitment in vivo. In addition, our data indicate retinal venous dilation in response to endotoxin. |
| Databáze: | OpenAIRE |
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