Identification of specific vascular endothelial growth factor susceptible and protective haplotypes associated with recurrent spontaneous miscarriages
| Autor: | Sondes Hizem, Safia A. Messaoudi, Wassim Y. Almawi, V. Herbepin, Maryam Dendana, K. Ben Jazia, Kalthoum Magdoud, Touhami Mahjoub, Renaud Touraine |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Vascular Endothelial Growth Factor A medicine.medical_specialty Abortion Habitual Tunisia Single-nucleotide polymorphism Biology Real-Time Polymerase Chain Reaction Polymorphism Single Nucleotide chemistry.chemical_compound Gene Frequency Internal medicine Genotype medicine Humans Genetic Predisposition to Disease Retrospective Studies Genetics Spontaneous miscarriage Rehabilitation Haplotype Obstetrics and Gynecology Minor allele frequency Vascular endothelial growth factor Vascular endothelial growth factor A Endocrinology Reproductive Medicine chemistry Haplotypes Case-Control Studies Female Gene polymorphism |
| Zdroj: | Human reproduction (Oxford, England). 27(5) |
| ISSN: | 1460-2350 |
| Popis: | BACKGROUND We investigated the association of vascular endothelial growth factor (VEGF) gene polymorphism with recurrent spontaneous miscarriage (RSM). METHODS VEGF -2578C/A, -1154G/A, -634G/C, +936C/T single nucleotide polymorphisms (SNPs) were assessed in 304 RSM patients, and 371 age-and body mass index-matched control subjects using real-time PCR. RESULTS Higher minor allele frequency of -1154G/A (P < 0.001) and +936C/T (P < 0.001), but not -2578C/A (P = 0.55) or -634G/C (P = 0.87) SNPs, were seen in patients. Significant differences in the distribution of -1154G/A (P = 0.006) and +936C/T (P = 0.015), but not -2578C/A (P = 0.473) or -634G/C (P = 1.000) genotypes, were seen in cases compared with control women. Of the possible 16 VEGF haplotypes, 9 were found to be common, and were included. A significantly lower frequency of C G C C (P = 0.008), and A G G C (P < 0.001) haplotypes, and a higher frequency C G C T (P = 0.020), and C G T (P = 0.004) haplotypes were seen in patients. CONCLUSIONS These results strongly support that VEGF polymorphisms, in particular-1154G/A and +936C/T, are significantly associated with RSM. Our results confirm, in the largest sample to date, previous works in other populations on VEGF polymorphism in RSM. |
| Databáze: | OpenAIRE |
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