Mosquito Feeding Assays to Determine the Infectiousness of Naturally Infected Plasmodium falciparum Gametocyte Carriers
| Autor: | Yimin Wu, Ashley J. Birkett, Patricia M. Graves, Mouctar Diallo, Louis Clément Gouagna, Thomas S. Churcher, Merribeth J. Morin, Mamadou B. Coulibaly, Bert Mulder, Teun Bousema, Robert W. Sauerwein, Chris Drakeley, Ogobara K. Doumbo, Will Roeffen, Rhoel R. Dinglasan, Isabelle Morlais, Vincent Robert, Yeya T. Touré, G. A. T. Targett, Parfait Awono-Ambene, Emily Locke, Timoléon Tchuinkam, Sarah Bonnet, Colin J. Sutherland, Travis van Warmerdam |
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| Přispěvatelé: | London School of Hygiene and Tropical Medicine (LSHTM), Radboud university [Nijmegen], Johns Hopkins Bloomberg School of Public Health [Baltimore], Johns Hopkins University (JHU), Institut de Recherche Agricole pour le Développement [Yaoundé] (IRAD), Institut de Recherche pour le Développement, Partenaires INRAE, Institut de Recherche pour le Développement (IRD), Malaria Res Inst, Biologie Moléculaire et Immunologie Parasitaires et Fongiques, École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Université des sciences, des techniques et des technologies de Bamako (USTTB), Laboratorium Microbiologie Twente, James Cook University (JCU), PATH Malaria Vaccine Initiat, NIAID, Imperial College London, Bill & Melinda Gates Foundation, European FP7 framework (REDMAL) [242079], PATH Malaria Vaccine Initiative (MVI), MVI, Bloomberg Family Foundation, Johns Hopkins Malaria Research Institute, Institut de Recherche pour le Developpement, TMRC, NIAID/NIH, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, EC FP7 Collaborative project TransMalariaBloc [HEALTH-F3-2008-223736], Radboud University [Nijmegen], École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Université des Sciences, des Techniques et des Technologies de Bamako (USTTB) |
| Rok vydání: | 2012 |
| Předmět: |
Male
moustique Epidemiology [SDV]Life Sciences [q-bio] lcsh:Medicine test cutané Skin infection Plasmodium 0302 clinical medicine Poverty-related infectious diseases Infection and autoimmunity [N4i 3] Blood plasma Malaria Falciparum lcsh:Science Child Whole blood 0303 health sciences Multidisciplinary biology Transmission (medicine) Anopheles Middle Aged maladie parasitaire 3. Good health skin infections Infectious Diseases Child Preschool Medicine Female Research Article Adult Adolescent Plasmodium falciparum 030231 tropical medicine malaria gametocyte Microbiology Infectious Disease Epidemiology Young Adult 03 medical and health sciences parasitic diseases Parasitic Diseases medicine Gametocyte sang Animals Humans Biology 030304 developmental biology Population Biology lcsh:R Infant plasma sanguin medicine.disease biology.organism_classification Virology infection Insect Vectors paludisme Immunology lcsh:Q Parasitology Malaria |
| Zdroj: | PLoS One, 7 PLoS ONE PLoS ONE, Public Library of Science, 2012, 7 (8), ⟨10.1371/journal.pone.0042821⟩ Plos One 8 (7), . (2012) PLoS ONE; Vol 7 PLoS ONE, 2012, 7 (8), ⟨10.1371/journal.pone.0042821⟩ PLoS One, 7, 8 PLoS ONE, Vol 7, Iss 8, p e42821 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Contains fulltext : 108727.pdf (Publisher’s version ) (Open Access) INTRODUCTION: In the era of malaria elimination and eradication, drug-based and vaccine-based approaches to reduce malaria transmission are receiving greater attention. Such interventions require assays that reliably measure the transmission of Plasmodium from humans to Anopheles mosquitoes. METHODS: WE COMPARED TWO COMMONLY USED MOSQUITO FEEDING ASSAY PROCEDURES: direct skin feeding assays and membrane feeding assays. Three conditions under which membrane feeding assays are performed were examined: assays with i) whole blood, ii) blood pellets resuspended with autologous plasma of the gametocyte carrier, and iii) blood pellets resuspended with heterologous control serum. RESULTS: 930 transmission experiments from Cameroon, The Gambia, Mali and Senegal were included in the analyses. Direct skin feeding assays resulted in higher mosquito infection rates compared to membrane feeding assays (odds ratio 2.39, 95% confidence interval 1.94-2.95) with evident heterogeneity between studies. Mosquito infection rates in membrane feeding assays and direct skin feeding assays were strongly correlated (p |
| Databáze: | OpenAIRE |
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