1274 full-open reading frames of transcripts expressed in the developing mouse nervous system
| Autor: | Jenny Li-Chun Lin, Jennifer L. Bair, Maria de Fatima Bonaldo, Anna I. R. De Melo, Joshua Rehmann, Val C. Sheffield, Jim J.-C. Lin, Mark Lebeck, Marcelo B. Soares, Todd E. Scheetz, Thomas L. Casavant, Brian Berger, Aja Davis, Dylan Tack, Tamara A. Kucaba, Ike Akabogu, Keith Crouch, Rebecca S. Reiter, Einat Snir, Mari E. Eyestone, Kelly Schaefer, Kurtis Trout, Thomas B. Bair, Catherine Keppel, Christina H. Smith |
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| Rok vydání: | 2004 |
| Předmět: |
clone (Java method)
DNA Complementary Nerve Tissue Proteins Biology Eye Genome Mice Open Reading Frames Pregnancy Genetics Animals Genomic library RNA Messenger Letters Genetics (clinical) Gene Library Messenger RNA cDNA library Gene Expression Profiling Brain Gene Expression Regulation Developmental Molecular biology Gene expression profiling Mice Inbred C57BL Open reading frame Cytoplasm Female |
| Zdroj: | Genome research. 14(10B) |
| ISSN: | 1088-9051 |
| Popis: | As part of the trans-National Institutes of Health (NIH) Mouse Brain Molecular Anatomy Project (BMAP), and in close coordination with the NIH Mammalian Gene Collection Program (MGC), we initiated a large-scale project to clone, identify, and sequence the complete open reading frame (ORF) of transcripts expressed in the developing mouse nervous system. Here we report the analysis of the ORF sequence of 1274 cDNAs, obtained from 47 full-length-enriched cDNA libraries, constructed by using a novel approach, herein described. cDNA libraries were derived from size-fractionated cytoplasmic mRNA isolated from brain and eye tissues obtained at several embryonic stages and postnatal days. Altogether, including the full-ORF MGC sequences derived from these libraries by the MGC sequencing team, NIH_BMAP full-ORF sequences correspond to approximately 20% of all transcripts currently represented in mouse MGC. We show that NIH_BMAP clones comprise 68% of mouse MGC cDNAs > or =5 kb, and 54% of those > or =4 kb, as of March 15, 2004. Importantly, we identified transcripts, among the 1274 full-ORF sequences, that are exclusively or predominantly expressed in brain and eye tissues, many of which encode yet uncharacterized proteins. |
| Databáze: | OpenAIRE |
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