Improvement of the ammonia measurement using a clarifying reagent and application to evaluate heat damage in commercial milk samples
| Autor: | Jean-Luc Gaillard, Gérard Humbert, Marie-France Guingamp |
|---|---|
| Přispěvatelé: | Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) |
| Rok vydání: | 2007 |
| Předmět: |
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
LACTULOSE 01 natural sciences Applied Microbiology and Biotechnology High-performance liquid chromatography Absorbance Ammonia chemistry.chemical_compound Lactulose Capillary electrophoresis medicine Ammonia Measurement CLARIFYING REAGENT AMMONIA Chromatography 010401 analytical chemistry ABSORBANCE A340 0402 animal and dairy science food and beverages HEAT DAMAGE 04 agricultural and veterinary sciences Repeatability 040201 dairy & animal science 0104 chemical sciences chemistry Reagent METHODE ELECTRODES SPECIFIQUES Food Science medicine.drug |
| Zdroj: | International Dairy Journal International Dairy Journal, Elsevier, 2007, 17 (8), pp.902-906. ⟨10.1016/j.idairyj.2006.12.009⟩ |
| ISSN: | 0958-6946 |
| DOI: | 10.1016/j.idairyj.2006.12.009 |
| Popis: | International audience; The enzymatic Boehringer procedure for ammonia measurement in milk was improved to be rapid and simple using a clarifying reagent without any pretreatment of the sample. A good correlation (r=0.99, n=36) existed between the proposed enzymatic method (EM) and the specific electrode method (SEM) when applied on raw and commercial milk samples. The repeatability of the EM applied to UHT milk was satisfactory with a relative standard deviation of 5.4% (n=26). The EM was used to evaluate heat damage in commercial half-skimmed pasteurised, direct or indirect or unlabelled UHT and in-bottle sterilised milk. The EM was well correlated with absorbance at 340 nm (A340) of transparent modified milk (r=0.97, n=42), with lactulose determined by HPLC and by capillary electrophoresis (r=0.95, n=17 and r=0.95, n=26, respectively). All parameters differentiated significantly (P |
| Databáze: | OpenAIRE |
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