Molecular analysis and nucleotide sequence of finQ, a transcriptional inhibitor of the F plasmid transfer genes
| Autor: | Lindsay M. Ham, Ron Skurray |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Gel electrophoresis
DNA Bacterial Base Sequence Molecular Sequence Data Nucleic acid sequence Molecular cloning Biology Transfection Molecular biology DNA-Binding Proteins Plasmid Restriction map Genes Bacterial Mutation Genetics DNA Transposable Elements Escherichia coli Amino Acid Sequence Cloning Molecular Site-directed mutagenesis Molecular Biology Gene Peptide sequence Plasmids Transcription Factors |
| Zdroj: | Moleculargeneral genetics : MGG. 216(1) |
| ISSN: | 0026-8925 |
| Popis: | We report the cloning of finQ, a gene coding for fertility inhibition of the F plasmid, from the IncI R factor R820a. The finQ gene was mapped precisely within a 1.24 kb region by ptac-transposase + min-kan mutagenesis and its product, FinQp, identified as a single polypeptide by means of SDS-polyacrylamide gel electrophoresis. Nucleotide sequencing of the finQ region allowed elucidation of the FinQp amino acid sequence and determination of its precise molecular weight as 39,895 Da. Analysis of the predicted amino acid sequence indicated that FinQp is a positively charged protein possessing a helix-turn-helix DNA binding motif. We propose a possible model for the mechanism by which FinQp terminates transcription within the F plasmid tra region. DNA-DNA hybridization established that all FinQ+ R factors examined have an homologous finQ gene. |
| Databáze: | OpenAIRE |
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