Pertussis toxoid structure: a collaboration and comparison of two-dimensional liquid chromatography-tandem mass spectrometry, ultraperformance liquid chromatography-mass spectrometryE, and capillary liquid chromatography-matrix-assisted laser desorption ionization-tandem mass spectrometry
| Autor: | Manorama Tummala, Almary Chacon, Edward K. Chess, Peifeng Hu, Shwu-Maan Lee |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
Chromatography Capillary action Molecular Sequence Data Biophysics Analytical chemistry Peptide Cell Biology Toxoids Tandem mass spectrometry Mass spectrometry Biochemistry Matrix-assisted laser desorption/ionization chemistry Liquid chromatography–mass spectrometry Tandem Mass Spectrometry Desorption Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Data-independent acquisition Amino Acid Sequence Molecular Biology Chromatography High Pressure Liquid |
| Zdroj: | Analytical biochemistry. 437(1) |
| ISSN: | 1096-0309 |
| Popis: | The overall structure of pertussis toxoid has been established by analysis of its tryptic digest using two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS), capillary liquid chromatography-matrix-assisted laser desorption ionization-tandem mass spectrometry (CapLC-MALDI-MS/MS), and ultraperformance liquid chromatography-mass spectrometry(E) (UPLC-MS(E)). In addition to oxidation and hydrolysis of amino acids losses of terminal peptides are observed. On-line UPLC-MS(E) generated a similar sequence coverage as the other two methods that involved off-line fraction collection. In light of recent favorable comparisons to data-dependent acquisition, UPLC-MS(E) should be the initial method of choice for analysis of a peptide mixture of moderate complexity. |
| Databáze: | OpenAIRE |
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