The meiosis-specific MEIOB-SPATA22 complex cooperates with RPA to form a compacted mixed MEIOB/SPATA22/RPA/ssDNA complex

Autor: Pauline Dupaigne, Carole Saintomé, Didier Busso, Cécile Ducrot, Gabriel Livera, Clotilde Duquenne, Jonathan Ribeiro, Xavier Veaute, Emmanuelle Martini, Raphael Guerois, Cynthia Petrillo
Přispěvatelé: Stabilité génétique, cellules souches et radiations (SGCSR (U_1274 / UMR_E_008)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay-Université Paris Cité (UPCité), Muséum national d'Histoire naturelle (MNHN), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2020
Předmět:
Models
Molecular
Protein Conformation
DNA
Single-Stranded
Cell Cycle Proteins
Biology
complex mixtures
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Meiosis
Cell Line
Tumor
Replication Protein A
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Animals
Humans
Homologous chromosome segregation
SsDNA binding
Crossing Over
Genetic
Homologous Recombination
Molecular Biology
Mitosis
030304 developmental biology
0303 health sciences
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Structural Biology [q-bio.BM]
Cell Biology
Key features
Cell biology
DNA-Binding Proteins
enzymes and coenzymes (carbohydrates)
Chromosome Pairing
HEK293 Cells
chemistry
030220 oncology & carcinogenesis
Multiprotein Complexes
Homologous recombination
DNA
Zdroj: DNA Repair
DNA Repair, 2021, 102, pp.103097. ⟨10.1016/j.dnarep.2021.103097⟩
ISSN: 1568-7856
1568-7864
Popis: During meiosis, programmed double-strand breaks are repaired by homologous recombination (HR) to form crossovers that are essential to homologous chromosome segregation. Single-stranded DNA (ssDNA) containing intermediates are key features of HR, which must be highly regulated. RPA, the ubiquitous ssDNA binding complex, was thought to play similar roles during mitotic and meiotic HR until the recent discovery of MEIOB and its partner, SPATA22, two essential meiosis-specific proteins. Here, we show that like MEIOB, SPATA22 resembles RPA subunits and binds ssDNA. We studied the physical and functional interactions existing between MEIOB, SPATA22, and RPA, and show that MEIOB and SPATA22 interact with the preformed RPA complex through their interacting domain and condense RPA-coated ssDNA in vitro. In meiotic cells, we show that MEIOB and SPATA22 modify the immunodetection of the two large subunits of RPA. Given these results, we propose that MEIOB-SPATA22 and RPA form a functional ssDNA-interacting complex to satisfy meiotic HR requirements by providing specific properties to the ssDNA.
Databáze: OpenAIRE
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