Mapping biological composition through quantitative phase and absorption X-ray ptychography
| Autor: | Michael T. Ryan, Andrew G. Peele, Grant van Riessen, Kirstin Elgass, Michael W. M. Jones, Mac B. Luu, Mark D. Junker |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Materials science Phase (waves) Contrast Media Electrons Article law.invention Mice Optics Optical microscope law Microscopy medicine Animals Projection (set theory) Absorption (electromagnetic radiation) Multidisciplinary business.industry X-Rays Fibroblasts Sample (graphics) Ptychography Molecular Imaging Cell Tracking Molecular imaging business |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Isolating compositional information in biological X-ray imaging can be problematic as such information is conflated with thickness and density variations when viewing in projection through a sample. We demonstrate an effective method for identifying variations in material composition by simultaneously using the quantitative phase and magnitude images provided through soft X-ray ptychography. Using this approach we show significantly increased contrast and improved reliability of the identification of intracellular features from uncharacterised samples. While demonstrated for X-ray ptychography, this method is immediately applicable to electron and optical microscopy methods where the complex transmission function of the sample is recovered. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|