Panorama of ancient metazoan macromolecular complexes
| Autor: | Zuyao Ni, Edward M. Marcotte, W. Brent Derry, Greg W. Clark, Snejana Stoilova, John B. Wallingford, John Parkinson, Alexandr Bezginov, Kevin Drew, Ramy H. Malty, Andrew Emili, Daniel R. Boutz, Blake Borgeson, Elisabeth R. M. Tillier, Fan Tu, Jack Greenblatt, Graham L. Cromar, Mihail Sarov, Kyle Chessman, Olga Kagan, Cuihong Wan, Xinghua Guo, Mohan Babu, Sadhna Phanse, Swati Pal, Xuejian Xiong, Pierre C. Havugimana, Julian Kwan, Ophelia Papoulas |
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| Rok vydání: | 2015 |
| Předmět: |
Biochemical fractionation
Systems biology Datasets as Topic Zoology Biology Genome Article Evolution Molecular 03 medical and health sciences 0302 clinical medicine Extant taxon Tandem Mass Spectrometry Protein Interaction Mapping Animals Humans Protein Interaction Maps 030304 developmental biology 0303 health sciences Modularity (networks) Multidisciplinary Systems Biology Reproducibility of Results Multicellular organism Evolutionary biology Multiprotein Complexes Macromolecular Complexes 030217 neurology & neurosurgery Protein Interaction Map |
| Zdroj: | Nature |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/nature14877 |
| Popis: | Macromolecular complexes are essential to conserved biological processes, but their prevalence across animals is unclear. By combining extensive biochemical fractionation with quantitative mass spectrometry, here we directly examined the composition of soluble multiprotein complexes among diverse metazoan models. Using an integrative approach, we generated a draft conservation map consisting of more than one million putative high-confidence co-complex interactions for species with fully sequenced genomes that encompasses functional modules present broadly across all extant animals. Clustering reveals a spectrum of conservation, ranging from ancient eukaryotic assemblies that have probably served cellular housekeeping roles for at least one billion years, ancestral complexes that have accrued contemporary components, and rarer metazoan innovations linked to multicellularity. We validated these projections by independent co-fractionation experiments in evolutionarily distant species, affinity purification and functional analyses. The comprehensiveness, centrality and modularity of these reconstructed interactomes reflect their fundamental mechanistic importance and adaptive value to animal cell systems. |
| Databáze: | OpenAIRE |
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